Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the stain affect the staining of bacteria? Can dyes other than methylene blue be used for direct staining? Briefly explain.
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- Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?Is it possible to perform double or triple staining using fluorescent dyes? Explain.Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram positive and negative cells. This being the case, explain how they end up staining Gram positive and gram negative cells differently.
- For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts). Stain Cationic or Anionic Dye malachite green crystal violet nigrosin safraninDescribe the procedure for performing a Gram stain. What is the procedure? How does each component work? (ie. What does the mordant do?)What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain, what is the mordant?
- Is acid fast staining differential stain , if yes, what are the 2 types of stain usedWhich type of stain would be the best choice for negative staining of most bacterial cells: a basic stain or an acidic stain?Briefly explain the difference between Wright Staining and H&E Staining (why use one over the other)
- What is the difference between simple and differential stains? Give an example of a differential stain.Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayWhat is the secondary stain in both acid-fast stain methods?