More Information on Chem21Labs graphs. Slope = 1452.0.... Yint = -0.021856.... R? = 0.99662 Mouse Coordinates: Absorbance vs Concentration 0.8 0.7 0.6 0.5 04 03 0.2 01 0- 0.00005 0.00010 0.00015 0.00020 0.00025 0.00030 0.00035 0.00040 000045 0.00050 0 00055 Concentration (M) Report the Slope of the best-fit line for the Calibration Curve NG 0.25 Info Absorbance
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- 3. If you wished to study the molecule below by measuring absorbance, what wavelength should you set your spectrophotometer to? 1.6 Absorbance 1.4 1.2 1.0 0.8 0.6 0.4- 0.2- ME 0.0 300 400 600 Wavelength (nm) 500 Solutions and Mol....pdf 2 700 20221027_141317.jpg 800Briefly describe how a phase-contrast microscope work and the kind of image that itproduces. Give a specific use for this type of microscope.Concentration [M] Absorbance Solution 1 86.29 0.64 Solution 2 51.986 0.29 …. Fig 1: [graph of the calibration curve, make sure to show: function, R2, 0 intercept]
- Yellow Dye Calibration Curve y = 254.78x - 0.1414 R? = 0.9792 2.5 1.5 1 0.5 0.002 0.004 0.006 0.008 0.01 0.012 Concentration(M) 3. 2. AbsorbanceCompute for the linearity (r2) value of the following concentration and absorbance values: SAMPLE CONCENTRATION ABSORBANCE 1 0.178 0.2127 2 0.274 0.2715 3 0.473 0.3892 4 0.574 0.4568 5 0.673 0.5327 6 0.864 0.6283 O A. 0.9962 B. 0.9976 OC. 0.9986 D. 0.9876Convert the following absorbance to transmittance. a. 0.283 b. 0.625
- Use least square regression to determine the best straight line and slope; compare the slope from 5 & 6. Concentration (M) Absorbance (no units) 0.10 0.09 0.20 0.14 0.30 0.19 0.40 0.27 0.50 0.30 0.60 0.34 0.70 0.37Given the calibration curve below, what is the limit of linearity? Absorbance 2000 1800 1600 1400 1200 1000 800 600 400 200 0 Ο 0.2 μg/ml Ο 0.4 μg/ml Ο 0.8 μg/ul Ο 1.2 μg/ul 0 Non-linear regresssion ...... 1 Concentration µg/μl 0.2 0.4 0.6 0.8 R²=0.9966 1.2 1.4 1.6Express the absorbance of 0.66 in terms of percent transmittance. A. 66% В. 22% С. 78% D. 34% E. 33%
- Which data point in your standard curve is the most important in determining the best straight line 1. through your data? Why? Which is the second most important? Why? pathlength cell displays % T of 50.0 what is the Absorbance of the solution and what is the absorptivity (in /cm-mole). What Absorbance would be expected for a 5.0 x 10-6 M solution? What % T would this correspond to? 2. A solution of a light absorbing species with a concentration of 1.0 x 10-5M in a 1.0 cmConcentration of FeSCN2+ Absorbance (Y axis) Percent Transmittance (%T) (X axis) 100 1.00E-04 0.495 32 2.00E-04 0.955 11.1 3.00E-04 1.398 Calibration Curve (Absorbance vs. Concentration of FESCN²*) 1.6 1.4 y = 4713.6x. 1.2 1 0.8 0.6 0.4 0.2 0.00005 0.0001 0.00015 0.0002 0.00025 0.0003 0.00035 Concentration of FeSCN²+ AbsorbanceUsing this table for the standard durve of cobalt (II) solution in UV Vis Molarity (M) Absorbance 0.025 0.056 0.05 0.131 0.075 0.221 0.1 0.311 0.125 0.399 0.15 0.492 0.175 0.592 Determine the (3 sig figs) A. R squared value of the curve B. value of the molar absorptivity constant if the path length is 10 cm? C. Unknown concentration if it showed an absorbance value of 0.320?