Net Absorbance (595 nm) Which protein standard curve would you use to read off the protein concentration of an unknown sample with an absorbancy value 175 150 125 .00 0.75 0.50 0.25 BSA BGG 0.00 500 1,000 1,500 2,000 Protein Concentration (pg/ml) Oouad imn BSA standard curve O BGG standard curve O Ether BSA or BGG standard curves O Neither BSA or BGG standard curves QUESTION 2

Net Absorbance (595 nm) Which protein standard curve would you use to read off the protein concentration of an unknown sample with an absorbancy value 175 150 125 .00 0.75 0.50 0.25 BSA BGG 0.00 500 1,000 1,500 2,000 Protein Concentration (pg/ml) Oouad imn BSA standard curve O BGG standard curve O Ether BSA or BGG standard curves O Neither BSA or BGG standard curves QUESTION 2

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter1: Introduction

Section: Chapter Questions

Problem 1.11QAP

See similar textbooks

Similar questions

You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.523 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.251 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
A solution of Tryptophan has an absorbance at 280 nm of 0.54 in a 0.5 cm length cuvette. Given the absorbance coefficient of trp is 6.4 x 103 Lmol-1cm-1. What is the concentration of solution? The answers are as follows A)0.17x10-3mol/L B)2.57x10-3mol/L C)0.17x10+3mol/L D)2.57x10+3mol/L
A solution of Tryptophan has an absorbance at 280 nm of 0.54 in a 0.5 cm length cuvette. Given the absorbance coefficient of trp is 6.4 x 103 Lmol-1cm-1. What is the concentration of solution? The options are as follows A) 0.17 x10-3 mol/L B)2.56x10-3mol/L C)0.17x10+3mol/L D)2.56x10+3mol/L
A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.144. Assuming that the best fit linear line of the standard curve was y=0.04144x+0.01521 (μgmL), calculate the percent protein by mass in the original protein powder.
Calculate the concentration of a solution of peptide AWSME, in mM if the absorbance at 280 nm for the solution is 0.6 absorbance units, and a 0.2-cm cuvette is used. For the extinction coefficient, take into account that e280(Trp) = 5690 M-1 cm-1 and e280(Tyr) = 1280 M-1 cm-1. Round your answer to two decimals.
Please complete Table 2 and show the calculations for protein concentration and the concentration of the undiluted sample. a). Using Excel software, enter data from Table 1 to plot a standard curve (corrected average absorbance on Y-axis and protein concentrations on X-axis). The standard curve must contain correct labels on both axes. b). From the standard curve, obtain a best fit line and equation. c). Use the best fit equation and data from Table 2 to find the concentrations of proteins in sample A2-D2 and E1. [onto your plotted standard curve, indicate where A595 of A2-D2 and E1 are located] d). Calculate the concentrations of proteins in undiluted samples A to E. Thank you very much.
The following data were obtained from a Bradford assay to determine the protein content in a sample: Protein content (μg) Absorbance value 0 0.000 2 0.022 5 0.065 10 0.106 20 0.178 30 0.299 40 0.380 50 0.472 unknown 0.150 a. use excel, another program, or graph paper to produce a calibration curve based on the data. b. Use linear regression (best fit line) to obtain the slope of the line c. Determine the unknown protein content for a sample that shows an absorbance reading of 0.150.
A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.187. Assuming that the best fit linear line of the standard curve was y = 0.04144 x + 0.01521 (μ g mL), calculate the percent protein by mass in the original protein powder.
Monomer-dimer formation can affect the absorbance measurements using Beer’s law, it is an example of: A. chemical deviation B. instrumental deviation C. real deviation D. instrumental deviation
The absorbance for a dye sample of unknown concentration is measured and the corrected absorbance is 0.666. The standard curve created is found to have this linear equation: y = 0.153x + 0.043 Calculate the concentration (ug / mL) of the dye sample. 4.54 3.33 4.07 5.17 2.07
A calibration plot of absorbance vs concentration (ppm) was obatined with standard known Red 40 dye solution. - the slope of the best-fit straight line of the plot is 0.057 ppm^-1 - The absorbance of the dilute unknown sports drink was 0.88 What is the concentration of this dilute unknown sports drink?______ the answer is 15.4 ppm ( can you please show me how to calculate this problem)