Only five general types of reactions are commonly observed in biochemical transformations. Given the proposed mechanism (illustrated above) for Class A β-lactamases, what is the biochemical reaction type represented by steps 1 and 2? O oxidation/reductionO carbonyl condensationO a, B-eliminationnucleophilic acyl substitutionnucleophilic addition P. aeruginosa resistance to B-lactam antibioticsThe most alarming finding with this strain of P. aeruginosa is its newly acquired resistance to a wide range of B-lactam antibiotics. Bacterial resistance to 8-lactams is derived fromenzymes hydrolyze the lactam ring of the antibiotic, rendering it useless. A typical mechanism forclass of enzymes known as 8-lactamases. TheseClass A 3-lactamase enzyme is illustrated:RH-o Ser0= HNSTEP ISTEP 2SerNH,NH,GluLysGluH20STEP 3STEP 5RноSerSTEP 4HNноSerSerGluNH,

Class A β-lactamases are enzymes that hydrolyze its substrate β-lactam; by forming an acyl-enzyme…

Only five general types of reactions are commonly observed in biochemical transformations. Given the proposed mechanism (illustrated above) for Class A β-lactamases, what is the biochemical reaction type represented by steps 1 and 2?

Only five general types of reactions are commonly observed in biochemical transformations. Given the proposed mechanism (illustrated above) for Class A β-lactamases, what is the biochemical reaction type represented by steps 1 and 2?

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter18: Glycolysis

Section: Chapter Questions

Problem 18P: Distinguishing the Mechanisms of Class I and Class I Aldolases Fructose bisphosphate aldolase in...

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Question

O oxidation/reduction
O carbonyl condensation
O a, B-elimination
nucleophilic acyl substitution
nucleophilic addition

P. aeruginosa resistance to B-lactam antibiotics
The most alarming finding with this strain of P. aeruginosa is its newly acquired resistance to a wide range of B-lactam antibiotics. Bacterial resistance to 8-lactams is derived from
enzymes hydrolyze the lactam ring of the antibiotic, rendering it useless. A typical mechanism for
class of enzymes known as 8-lactamases. These
Class A 3-lactamase enzyme is illustrated:
R
H-o Ser
0= HN
STEP I
STEP 2
Ser
NH,
NH,
Glu
Lys
Glu
H20
STEP 3
STEP 5
R
но
Ser
STEP 4
HN
но
Ser
Ser
Glu
NH,

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