Phosphate buffered saline (PBS) is routinely used in the lab to wash cells that are being grown in dishes. Assuming intracellular concentrations are given in the table down below, calculate the Nernst Potential for Na+ and K+ of cells being washed by PBS.
Phosphate buffered saline (PBS) is routinely used in the lab to wash cells that are being grown in dishes. Assuming intracellular concentrations are given in the table down below, calculate the Nernst Potential for Na+ and K+ of cells being washed by PBS.
Chapter16: Applications Of Neutralization Titrations
Section: Chapter Questions
Problem 16.8QAP
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Question
Phosphate buffered saline (PBS) is routinely used in the lab to wash cells that are
being grown in dishes. Assuming intracellular concentrations are given in the table down below,
calculate the Nernst Potential for Na+ and K+ of cells being washed by PBS. The recipe for
phosphate buffered saline is given below. (Note, for calcium chloride and magnesium chloride,
the amounts are listed as the hydrated salt. This means that for every unit of chloride salt, there
is associated water – two water molecules for calcium chloride and six for magnesium chloride –
that should be accounted for in the formula weight of the salt.
Transcribed Image Text:lon
Extracellular
Intracellular
Concentration (mM) Concentration (mM)
145
Na+
15
K*
5
150
Ca+2
1.8
104
100
5
Transcribed Image Text:Reagent
Amount
to add for 1L of PBS
NaCl
8 g
0.2 g
1.44 g
KCI
NazHPO4
0.24 g
ΚHΡΟ.
CaCl,•2H2O
0.133 g
MgCl2•6H2O
0.10 g
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