QUESTION 6 A series of experiments were performed in Neurospora crassa in order to define the tryptophan biosynthesis pathway. In one of these experiments, five tryptophan auxotrophs were isolated and tested for growth on tryptophan and five other known intermediates in the tryptophan biosynthesis pathway. The growth responses are shown below: Growth on Anthranilic Acid (AA) Growth on Indole Growth on Indole Glycerol Phosphate (IGP) Growth on Chorismic Acid (CA) Growth on N- Anthranilate (NA) Growth on Tryptophan Mutants: trp-1 trp-2 + + trp-3 + trp-4 + + + trp-5 + + + - + + + + + + Using the growth data in the above table, indicate the precursor (molecule that you start with) and the order of the intermediates in this pathway (please use exact spelling or abbreviation). Rxnl Rxn2 Rxn3 Rxn5 Tryptophan Rxn4 Indicate the mutant that is associated with the enzyme that catalyzes the reactions for tryptophan biosynthesis using the data given in the above table. Rxn1: Rxn2: Rxn3: Rxn4: Rxn5:

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QUESTION 6
A series of experiments were performed in Neurospora crassa in order to define the tryptophan biosynthesis pathway. In
one of these experiments, five tryptophan auxotrophs were isolated and tested for growth on tryptophan and five other
known intermediates in the tryptophan biosynthesis pathway. The growth responses are shown below:
Growth on
Anthranilic
Acid (AA)
Growth
on Indole
Growth on
Indole Glycerol
Phosphate (IGP)
Growth on
Chorismic Acid (CA)
Growth on N-
Anthranilate
(NA)
Growth on
Tryptophan
Mutants:
trp-1
trp-2
+
+
trp-3
+
trp-4
+
+
+
trp-5
+
+
+
-
+
+
+
+
+
+
Using the growth data in the above table, indicate the precursor (molecule that you start with) and the order of the
intermediates in this pathway (please use exact spelling or abbreviation).
Rxnl
Rxn2
Rxn3
Rxn5
Tryptophan
Rxn4
Indicate the mutant that is associated with the enzyme that catalyzes the reactions for tryptophan biosynthesis using the
data given in the above table.
Rxn1:
Rxn2:
Rxn3:
Rxn4:
Rxn5:
Transcribed Image Text:QUESTION 6 A series of experiments were performed in Neurospora crassa in order to define the tryptophan biosynthesis pathway. In one of these experiments, five tryptophan auxotrophs were isolated and tested for growth on tryptophan and five other known intermediates in the tryptophan biosynthesis pathway. The growth responses are shown below: Growth on Anthranilic Acid (AA) Growth on Indole Growth on Indole Glycerol Phosphate (IGP) Growth on Chorismic Acid (CA) Growth on N- Anthranilate (NA) Growth on Tryptophan Mutants: trp-1 trp-2 + + trp-3 + trp-4 + + + trp-5 + + + - + + + + + + Using the growth data in the above table, indicate the precursor (molecule that you start with) and the order of the intermediates in this pathway (please use exact spelling or abbreviation). Rxnl Rxn2 Rxn3 Rxn5 Tryptophan Rxn4 Indicate the mutant that is associated with the enzyme that catalyzes the reactions for tryptophan biosynthesis using the data given in the above table. Rxn1: Rxn2: Rxn3: Rxn4: Rxn5:
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