Recall the semi discontinuity in DNA replication. Is it biologically possible for DNA to undergo replication in vivo, without the lagging and the leading strands? In PCR, we also start with an antiparallel strand of DNA. Does this also mean that replication is semidiscontinuous in in vitro replication? PLEASE EXPLAIN COMPLETELY AND NOT JUST COPY PASTE ANSWERS FROM GOOGLE.

Recall the semi discontinuity in DNA replication. Is it biologically possible for DNA to undergo replication in vivo, without the lagging and the leading strands? In PCR, we also start with an antiparallel strand of DNA. Does this also mean that replication is semidiscontinuous in in vitro replication? PLEASE EXPLAIN COMPLETELY AND NOT JUST COPY PASTE ANSWERS FROM GOOGLE.

Biochemistry

9th Edition

ISBN:9781305961135

Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Chapter10: Biosynthesis Of Nucleic Acids: Replication

Section: Chapter Questions

Problem 23RE: RECALL Single-stranded regions of DNA are attacked by nucleases in the cell, yet portions of DNA are...

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RECALL Single-stranded regions of DNA are attacked by nucleases in the cell, yet portions of DNA are in a single-stranded form during the replication process. Explain.
In Polymerase Chain Reaction (PCR), the temperature is one of the most important parameters that could influence the efficiency of this technique. Each cycle of this reaction has its own specific temperature. For instance, the denaturation step possesses a temperature of 94 - 98 ℃ to ensure that the double stranded DNA is fully separated. (i) (ii) (iii) Why is the annealing temperature vital in this technique? Explain how will this temperature affects the efficiency of this reaction. Why is Hot Start PCR technique preferred by some researchers? If the primers you purchased possessed the following information. 5'-GGA AAC AGC TAT GAC CAT G-3' Calculate the melting temperature of this primer and estimate the annealing temperature of this primer.
1) What happened to the DNA at the different temperatures? How does Polymerase Chain Reaction exploit this property of DNA (Word count: 200) 2) Briefly outline the steps of DNA extraction as carried out in a lab for the purposes of sequencing, with reference to a published protocol from a company that supplies regents and kits for DNA extraction (e.g Qiagen, ThermoFischer, Invitrogen, or similar). Please Include the weblink for the published protocol you used as a reference
In DNA extracting. What is the purpose of clear shampoo in the DNA extraction buffer?
TRUE OR FALSE. a) The Sanger method of DNA sequencing follows the principle of complementarity just like in the replication process. b) Supercoiling whether positive or negative, can be experienced in the replication or transcription process.
Similarities between DNA agarose gels and SDS-PAGE are: Both methods use agarose gels. Both methods use electrical charge differences to separate molecules by size. Both methods determine the size of DNA fragments. All the given answers.
Considering DNA sequencing by the Sanger method. It is correct to say that: * A)In the traditional method, radioactively “labeled” primers are used, allowing their visualization in autoradiography. B)In the automated method, a single reaction is performed containing the four “labeled” dideoxynucleotides, each with a different fluorophore. C)In both traditional and automated methods, the fragments are resolved and interpreted according to their ionization state. D)In the automated method, di-deoxynucleotides “labeled” with the same fluorophore are used, thus allowing their interpretation based on graphs of fluorescence emission. E)Sequencing reactions can use mRNA molecules, as long as they have a polyA tail.
2) explain why it is far less important for Primase to have proofreading activity than it is for DNA polymerase. 60-word limit
BamHI site BamHI site kanT 3755,6p 784 bp PAMP 4539 bp 2332 pp PKAN 4207 bp HindllI site 1875 bp amp HindlII site Origins of replication You are using these two plasmids to make a recombinant DNA molecule. You want to make a molecule, that has two origins of replication and is amp but NOT kanR, using only one fragment from each plasmid. How large, in base pairs, will be your desired recombinant DNA molecule? O 6087 base pairs O 5630 base pairs O 2659 base pairs O 8746 base pairs 47 8 24 & 4. 8. 9. 4 5 6 RI T 13 H. 2 K FL
Can you help with 1a please HELPFUL INFORMATION: When performing classical Sanger or "dideoxy" sequencing, you set up 4 parallel reactions per template to be sequenced from a specific primer, with each of the four reactions containing a different dideoxynucleotide, and then the four reactions were run in a separate, adjacent lanes on a gel. 1a. Why couldn't you combine all 4 dideoxynucleotides with the primer and the template and do the whole reaction in one tube, and then run the set of fragments produced by the reaction mixture on a single lane in an acrylamide gel?
Recall that Meselson and Stahl used heavy nitrogen (N15) and light nitorgen (N14) to determine the mode of DNA replication. If Meselson and Stahl had observed two bands on their gel following only one round of replication, which replication model would have been supported by this observation? conservative model of replication this observation would not have supported any of their models dispersive model of replication semi conservative model of replication O O O O
DNA QUANTITATION If you have the extracted/purified DNA and will not be immediately loaded in the spectrophotomer, how can you maximize the storage life of DNA? What practices will maximize the quality of your DNA sample?