Refer to the description and table below to answer the three question that follow: Dissolved oxygen levels from 3 bottles of algae were recorded. The DO level of bottle #1 was recorded immediately after the algae was collected, the DO level of bottle #2 was recorded after sitting for 20 hours wrapped in tin-foil, and the DO level of bottle #3 was recorded after sitting for 20 hours under a lamp. A heat sink was placed over all of the bottles to ensure that temperature did not have an effect on the results. The following data was collected: Bottle Number D.O. Level 8 1 6 #1 #2 #3 4. How many units of oxygen were used for cell respiration by the algae?
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- THIS IS A MULTIPART QUESTION, PLEASE ANSWER ALL QUESTION Using the image: 5. Identify whether the organism is a green alga, a diatom, a dinoflagellate, Eubacterium, or a Cyanobacterium. Explain the characteristic(s) that led you to that identification. 6. a. What type of microscopy (brightfield, darkfield, or phase contrast) was to obtain the image? Explain how you can tell. b. Name a different type of microscopy would be also appropriate to use to visualize the organism in the image. How would the image change if you used that type of microscopy instead? 7. The image was taken using a microscope that has 10X, 40X, and 100X objectives. What is a possible magnification for the ocular lens on this microscope? Explain your response or show calculations.Select ALL THE TRUE statements about the organisms you can see in the picture. *400x total magnification used Group of answer choices 1.these organisms move with pseudopods 2.these organisms are unicellular 3.these organisms are autotrophs 4.these organisms absorb sunlight with pigments 5.these organisms are multicellular 6.these organisms are heterotrophsFor the picture below, answer the following questions: A. What is the shape of the bacteria? B. What is the arrangement of the bacteria? C. If this slide shows a Gram stain, what is the Gram stain reaction? If this slide does not show a Gram stain, please state that as your answer. ASM MicrobeLibrary.org Smith
- Determine the classification of this organism's oxygen requirement, growing under different oxygen concentrations: Plated on TSA, anaerobe jar: Light growth Plated on TSA, grown in incubator: light growth Inoculated into fluid thioglycolate broth: Heavy growth throughout the entire tube asap please.Bacteria: Pseudomonas aeruginosa Describe the colony morphology.Specify the ecological or clinical significance of your unknown. Include a fun fact on your unknown. Indicate the habitat and optimal environmental parameters including temperature, pH, and osmotic pressure.place the statements into the following groups: Gram-negative organisms might incorrectly stain purple, Gram-negative organisms might not be visible, Gram-positive organisms might incorrectly stain pink, and you may not be able to view any orgwnisms on the slide. Here are the statements: -you forgot the gram's iodine -you applied the decolorizer for too long -you forgot the decolorization step -you forgot to apply safranin. -you forgot the crystal voilet step -you did not heat fix the smear correctly.
- Answer the question briefly and concisely. The Mannitol salt agar (MSA) only allows the growth of halophiles (salt-loving microbes);nonhalophiles will not grow. Among the halophiles, mannitol fermenters will produce acid that turnsthe pH indicator yellow; mannitol nonfermenters leave the medium red. Onto MSA you inoculate ahalophilic mannitol fermenter and a halophilic mannitol nonfermenter. In this case, the medium isacting as (a) __________ medium(s).A. SelectiveB. DifferentialC. Selective and differentialD. EnrichedGiven the groupings of microorganisms, please indicate within the prescribe column whether these characteristics belongs to the group. Indicate your answer by writing Y if a characteristic and N if a non-characteristic. If the characteristic is not true to all members of the group, then write NYWhy is it important to monitor plankton? What measurements are recorded when collecting water samples for the plankton monitoring community science program and what instruments are used to collect those measurements? Why is it important for all plankton monitors to use the sample protocol for both sample collection and identification? What are the ways you can control the amount of light passing through your specimen? What is resolution and how is it improved? With which objective is it permissible to use the course adjustment knob? Why is it necessary to use the fine adjustment knob with higher magnification? Many of the plankton images were taken using the 40x objective on a compound microscope. What is the total magnification? What is the relationship between Cyanobacteria and the chloroplast of phytoplankton (algae)? List three ways to distinguish Cyanobacteria from phytoplankton (algae). How can you differentiate phytoplankton to zooplankton?
- How does the streak plate technique help in isolating individual colonies of bacteria? Why streak multiple times on one plate? 3. While observing a plate inoculated with bacteria you observe a white-tan fuzzy growth. What can you interpret from this observation? Explain how this outcome could’ve occurred. 4. What are 3 possible errors that could take place as you culture your samples which could lead to contamination? State 3 and explain why. Microbiology classUse the following diagram to explain how Pasteur’s swan-necked flasks prevent contamination of sterile broth the flasks.Describe what happens to the sterile broth (a) after it has beenallowed to cool as in (b). What happens to the broth after theflask has been tipped enough to let the broth come in contactwith the dust and microorganisms and is tipped back as in (c)?Summarize the terms used to characterize microbes based on their physical growth factors; 1. pH (three terms, pH value, organism example each) 2. Temperature (five terms, temperature ranges, organism example each) 3. Oxygen (five terms, description of growth, organism example each) 4. Salt (three terms, salt concentration, organism example each) Please type