Show calculations and describe the preparation of 1.0 L of a 0.25 M Cys-Pro buffer at pH 8.1. Have all required glassware, a 1.0 M solution of Cys-Pro in its fully deprotonated form, 1.0 M HCl, 1.0 M NaOH and deionized water.

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Acid Base Titration

An acid-base titration is the method of quantitative analysis for determining the concentration of an acid and base by exactly neutralizing it with a standard solution of base or acid having known concentration.