"The design of trans-Tango is modular, so it is not limited to tracing experiments. Since the readout of trans-Tango is transcription of a reporter gene, the system can be readily configured for functional experiments in which the activity of second-order neurons is altered (e.g., by incorporating optogenetic proteins as the reporter) or monitored (e.g., by incorporating calcium sensors as the reporter)." If there's NO genetic restriction (that means you can put as many trans- genetic elements in the target fly), is it possible to further advance the trans-Tango system, making the system capable of labeling "the third-order" neurons (means the neurons that connect with the second- order neurons; aka down-stream of the second-order neurons)? What additional genetic elements are required? Please give your best shot to answer these questions.

"The design of trans-Tango is modular, so it is not limited to tracing experiments. Since the readout of trans-Tango is transcription of a reporter gene, the system can be readily configured for functional experiments in which the activity of second-order neurons is altered (e.g., by incorporating optogenetic proteins as the reporter) or monitored (e.g., by incorporating calcium sensors as the reporter)." If there's NO genetic restriction (that means you can put as many trans- genetic elements in the target fly), is it possible to further advance the trans-Tango system, making the system capable of labeling "the third-order" neurons (means the neurons that connect with the second- order neurons; aka down-stream of the second-order neurons)? What additional genetic elements are required? Please give your best shot to answer these questions.

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter17: Instrumentation In Intensive Care Units

Section: Chapter Questions

Problem 6Q

See similar textbooks

Similar questions

What type of experiment can be performed to confirm that the C-terminal KDEL tetrapeptide is both necessary and sufficient in ER localization? (Necessary meaning that without KDEL, ER retrieval would not occur, and sufficient meaning that with KDEL, we would definitely see its effect (ER retrieval would occur))
Suppose you want to determine if excessive lipid ingestion altersgene transcription. Describe and explain all the steps you wouldcarry out to do a microarray-based study of the question. Includeyour choice of subjects and tissues to be investigated. (Thestatement of the problem to be studied—effects of “excessivelipid ingestion”—is deliberately vague, and you should refine itby specifying and explaining an operational procedure.
Certain hormones, such as epinephrine, can increase the levels ofcAMP within cells. Let’s suppose you pretreat cells with or withoutepinephrine and then prepare a cell extract that contains theCREB protein.You then use an electrophoretic mobility shift assay to analyzethe ability of the CREB protein to bind to a DNA fragmentcontaining a cAMP response element (CRE). Describe what theexpected results would be.
Resident ER proteins destined to remain in the lumen of the ER are marked by retrieval sequences and if these proteins are exported from the ER to the Golgi, they are recognized by a recycling receptor in the ERGIC or the Golgi and selectively returned to the ER. Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a b True False
Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. There are many biomedical engineering based approaches to detect CSCs. 1) What is the importance and advanatge of detecting CSCs? 2) What kind of systems have been developed to detect CSCs? Describe by giving examples. Please explain in detail with your own words.
Alternative splicing is a common mechanism for eukaryotes toexpand their repertoire of gene functions. At least one estimateindicates that approximately 50 percent of human genes usealternative splicing, and approximately 15 percent of diseasecausingmutations involve aberrant alternative splicing. Differenttissues show remarkably different frequencies of alternativesplicing, with the brain accounting for approximately 18 percentof such events. Why might some tissues engage in more alternative splicingthan others?
Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. There are many biomedical engineering based approaches to detect CSCs. Question: What kind of systems have been developed to detect CSCs? Describe by giving examples. Please explain with a few examples.
Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. There are many biomedical engineering based approaches to detect CSCs. Question: What is the importance and advanatge of detecting CSCs?
By means of a flowchart, show the possible jumps that a transposoncan make. Show the involvement of viruses in its movement.
One complication of making a transgenic animal is that thetransgene might integrate at random into the coding region, orthe regulatory region, of an endogenous gene. What might be theconsequences of such random integrations?
With regard to regulatory proteins and small effector molecules, explain the meaning of negative control and inducible
Two GPCR mutations (Mutant A and Mutant B) were tested for activity. One was confirmed as being a constitutively activating, gain of function GPCR mutation and one was confirmed as being a loss of function GPCR mutation. Which is which? Explain your answer.