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- In the tripple sugar fermentation test, why is the triple sugar iron agar called a multi-test system? list the concentrations of sugars in the media and the possible results due to fermentationIf you streak a sample from your nose onto a Mannitol salt agar plate and the medium turns yellow, what does that tell you? (choose all that apply) a. The organism is a yeast b. The organism ferments mannitol c. The organism is beta-hemolytic d. The organism is likely Staphylococus aureus e. The organism is acid tolerantdiscuss how the black precipitate is formed in the butt of the test tube during a tripple sugar iron fermentation test using triple sugar iron agar.
- Describe what the plate in each scenario looks like: A. Bacteria grows on mannitol salt agar and is negative for mannitol fermentation B. Bacteria grows on EMB agar and is positive for lactose fermentation C. Bacteria grows on MacConkey agar and is negative for lactose fermentationDuring the heat shock step, a student accidentally heated the cells at 42°C for 5 minutes. Only a few colonies were found on the agar plates at the end of the experiment. Explain the results.A bacterial organism is used to inoculate mannitol broth medium. Following incubation the broth is yellow. This indicates that the organism: A) can use lactose and glucose B) cannot use lactose C) can use mannitol D) cannot use mannitol
- a) explain in your own words how to do a nitrate reduction test to identify unknown bacteria b) explain in your own words how to perform a phenol red broth (prb) test to help identify unknown bacteriaWhy are the Methyl Red/Voges-Proskauer tests done after 48 hours of incubation?a) Readings taken at earlier time points may be different from readings taken at 48 hours b) Bacteria need 48 hours to process all the oxygen in the media c) Bacteria grow slowly in the MR/VP media d) K2HPO4 needs 48 hours to react with the acids produced by fermenting bacteria2. Gamma-hemolysis on a blood agar plate would likely appear: Select one: a. As no change in the medium b. Blue c. Yellow d. Green e. As a clearing.
- Why is the sterile technique (the flame sterilization of wire loop) followed during the fermentation lab simulation? A. To ensure mono cultivation of yeast cells. B. To ensure optimal pH for the yeast cells to grow. C. To remove unwanted compounds, such as ammonia, in the medium. D. To remove oxygen and maintain anaerobic conditions.Give two ways to prevent the diffusion of oxygen into the medium in this test.Food microbiology involves the following, except _______________ . a. fermentationb. western blottingc. HACCPd. yeast removale. spoilage
