Using the information in the table below, enter the correct pH that should be used to separate the following three amino acids by electrophoresis: (a) Cys. Tyr, and His (b) Gin, Ala, and Asp Val

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Using the information in the table below, enter the correct pH that should be used to separate the following three amino acids by electrophoresis:
(a) Cys, Tyr, and His
(b) Gin, Ala, and Asp
pk Values for Ionizable Groups of Amino Acids.
Amino Acid pk of a-COOH pk of a-NH3+ PK of Side Chain Isoelectric Point (pl)
Alanine
9.87
Arginine
2.01
9.04
Asparagine 2.02
Aspartic acid
Cysteine
Glutamic acid
Glutamine
Glycine
Histidine
Isoleucine
Leucine
Proline
Serine
Threonine
2.35
2.10
Submit Answer
2.05
2.10
2.17
2.35
1.77
Lysine
Methionine 2.28
Phenylalanine 2.58
2.32
2.33
2.18
2.00
2.21
2.09
Tryptophan 2.38
Tyrosine
2.20
Valine
2.29
8.80
9.82
10.25
9.47
9.13
9.78
9.18
9.76
9.74
8.95
9.21
9.24
10.60
9.15
9.10
9.39
9.11
9.72
Try Another Version
12.48
3.86
8.00
4.07
1
6.10
10.53
10.07
9 item attempts remaining
6.11
10.76
5.41
2.98
5.02
3.08
5.65
6.06
7.64
6.04
6.04
9.74
5.74
5.91
6.30
5.68
5.60
5.88
5.63
6.00
Transcribed Image Text:Using the information in the table below, enter the correct pH that should be used to separate the following three amino acids by electrophoresis: (a) Cys, Tyr, and His (b) Gin, Ala, and Asp pk Values for Ionizable Groups of Amino Acids. Amino Acid pk of a-COOH pk of a-NH3+ PK of Side Chain Isoelectric Point (pl) Alanine 9.87 Arginine 2.01 9.04 Asparagine 2.02 Aspartic acid Cysteine Glutamic acid Glutamine Glycine Histidine Isoleucine Leucine Proline Serine Threonine 2.35 2.10 Submit Answer 2.05 2.10 2.17 2.35 1.77 Lysine Methionine 2.28 Phenylalanine 2.58 2.32 2.33 2.18 2.00 2.21 2.09 Tryptophan 2.38 Tyrosine 2.20 Valine 2.29 8.80 9.82 10.25 9.47 9.13 9.78 9.18 9.76 9.74 8.95 9.21 9.24 10.60 9.15 9.10 9.39 9.11 9.72 Try Another Version 12.48 3.86 8.00 4.07 1 6.10 10.53 10.07 9 item attempts remaining 6.11 10.76 5.41 2.98 5.02 3.08 5.65 6.06 7.64 6.04 6.04 9.74 5.74 5.91 6.30 5.68 5.60 5.88 5.63 6.00
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