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- What are the differences of the 4 objective lenses of microscope?
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How to get the toal magnification of a specimen that is being observed?
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- Rowena was tasked to measure the actual magnification of her specimen mounted on a slide without drawing or taking a photomicrograph. Which type of microscope should she use? Why? How does it help her get the actual magnification?What is the purpose of using microscopes? If you need to move the microscope, describe how you should carry it. What three things change as you increase magnification? On the compound microscope, what power must be in place when you first find an image in themicroscope? On the compound microscope, which adjustment knob is used for bringing the specimen intofocus? On the compound microscope, which adjustment knob is used for sharpening the image of thespecimen after it is focused? On the compound microscope, which adjustment knob should you use on the high power? On the compound microscope, how do you change the objective lenses? What should be included when sketching or drawing what is seen through the microscope How do you properly use a microscope?Which of the following statements are true in the context of the microscope's field of view? I. It is the observable area within a circular space as defined by the lenses II. FOV is the smallest on the lowest power objective III. FOV is the maximum area visible through the microscope eyepiece IV. When you switch to a higher power, the field of zooms out towards the center
- What is the difference between a phase-contrast and differential interference-contrast microscope? When do you use each type?What’s the aim of using a microscope for examining? What can the kohler illumination technique tell us? What are the sources of error that can impact the data ?briefly describe the functions of the following parts of the microscope: course objective knob, condenser lens, diaphragm, objective lens, illuminator, fine adjustment knob, oil immersion.
- What are the advantages of using super-resolution microscopyversus standard fluorescence microscopy?How will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensityUsing the microscope Answer the following questions as you work through this exercise: 1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?
- What advantage do fluorescent dyes and fluorescence microscopy provide in comparison to the chemical dyes used to stain specimens for light microscopy?If a specimen is being viewed under a 40x objective, and the microscope has a 10x ocular lens, what is the total magnification? 1x 4x 10x 100x 400xAn object found with the 10x objective of the microscope may be viewed with the 40x objective without having to re-focus. This property of the microscope is called: O Confocal O Bifocal Binocular d Parfocal