Q: What are the 5 gene in order on the E. coli tryptophan operon?
A: The trp operon, found in the bacterium Escheichia coli is a group of genes that encode biosynthetic…
Q: What are the main elements of the lac operon and their functions?
A: Operon is the the prokaryotic gene regulatory system in which the expression of polycistronic mRNA…
Q: How does repression work in the lac operon?
A: Lac operon consists of a promoter, operator group of structural genes such as lac A, lac Z, and lac…
Q: What turns the operon on when lactose is present?
A: An operon is defined as a functioning unit of DNA that contains a cluster or group of genes under…
Q: Describe briefly the Trp operon? Why is it considered an example of negative regulation?
A: An operon is a cluster of genes that are transcribed together to give a single messenger RNA (mRNA)…
Q: What do you mean by coordinate expression of genes in operon?
A: Answer- In prokaryotes genes are controlled by one promoter region that is called polycistronic…
Q: Describe the structure of the Lac operon. How is it turned on? How is it turned off?
A: The gene products of the lac operon are very important for lactose metabolism. This is crucial for…
Q: What is the function of the Z gene of the Lac operon?
A: The lactose is cleaved into glucose and galactose by the enzyme β-galactosidase which is coded by…
Q: What is the difference between inducible and repressible operons?
A: The region of DNA which contains cluster of genes are known as operons. They are found in…
Q: What is an operon? Explain an inducible operon.
A: In genetics, an operon is a functioning unit of DNA containing a cluster of genes under the control…
Q: What is antisense RNA? How does it control gene expression?
A: RNA (ribonucleic acid) is a polymer of nucleotides and is composed of a pentose sugar, a nitrogen…
Q: The trp operon is a repressible operon system. What does this mean?
A: Trp refers to tryptophan. The operon is the functional unit of Deoxyribonucleic acid ( DNA) having…
Q: operon
A: Answer: Operon: In genetics, an operon is a functioning unit of DNA containing a cluster of genes…
Q: Why is the TRP operon considered a repressible operon, while the LAC operon is considered an…
A: Operons are areas of DNA which are composed of cluster of related genes which are controlled by…
Q: How is glucose involved in positive control of the lac operon?
A: A set of genes present in E.coli. that regulates the uptake of lactose from the surrounding…
Q: What is the function of each of the parts of the lac operon (promoter, operator, CAP binding site,…
A: The lactose operon (also called the lac operon) is a group of genes present in E. coli and other…
Q: What type of mutation in an operon is most likely to affect the synthesis of more than one protein?
A: Mutation is the process, in which genetic sequence is changed, which results in the change in…
Q: Explain the The Operon Model: Negative Control ?
A: The lac operon of E. coli contains genes involved in lactose metabolism. It is expressed only when…
Q: How does the lac operon help E. coli conserve energy and other resources?
A: Escherichia coli, also known as E. coli, is a Gram-negative, facultatively anaerobic, rod-shaped,…
Q: Which enzyme will be produced in a cell where a nonsense mutation is present in the lac operon?
A: In genetics, the mutation in which a sense codon that corresponds to one of the twenty amino acids…
Q: Give the Genetic Proof of the Operon Model ?
A: An operon represents a genetic unit with one or more structural genes and an operator sequence and…
Q: How do we know that the trp operon is a repressible controlsystem, in contrast to the lac operon,…
A: Introduction The process by which the information contained in a gene is used to drive the assembly…
Q: What led researchers to conclude that a repressor moleculeregulates the lac operon?
A: Lac operon is regulated by the lac repressor molecule.
Q: What is the order of ZYA on the lac operon?
A: Lac operon includes a set of genes having a single promoter, which enables the bacterium to secrete…
Q: What are the example of operon?
A: Operon is a group of genes which controls the synthesis process of protein molecules depending on…
Q: What is the major difference between the mechanism involved inattenuation and riboswitches and the…
A: Attenuation is a mechanism that controls gene expression in a bacterial operon and causes premature…
Q: What is a negative inducible operon?
A: Regulated genes can be switched on/off depending on cell's metabolic needs. Operon It is a group of…
Q: What is the difference between positive regulation of the lac operon and negative regulation of the…
A: The lac operon is a set of three bacterial genes under the control of a single promoter which is…
Q: How does the level of tryptophan alter transcription of the trp operon?
A: An operon is defined as the set of structural genes that are under the regulation of a common…
Q: The transcription of many bacterial genes relies on functional groups called operons, such as the…
A: Turning/switching genes on and off is referred to as gene regulation. Cells start to take on…
Q: How does cAMP and allolactose work to influence the operon?
A: The two proteins involved regulation of lac operon are CRP (catabolite repressor protein) and lac…
Q: What is not a part of an operon?
A: Operon comprises all the genes that are involved in metabolic regulation. It is the unit of the gene…
Q: How is the operon positively and negatively regulated?
A: The activity of the promoter that controls the expression of the lac operon is regulated by two…
Q: appropriate to use inducible operons? When is it appropriate to use repressible operons?
A: An operon is a collection of genes with similar functions that is managed by a single shared…
Q: What advantages are there to having genes arranged in an operon, compared with the arrangement in…
A: The gene is the basic morphological and physiological unit of heredity. DNA is used to make genes.…
Q: Can you think for how long the lack operon would be expreesed in the presence of lactose?
A: Lac operon is an operon that regulates the breakdown of lactose present in the medium. It contains…
Q: What is the difference between positive and negative control? What is the difference between…
A: The cluster of genes that are regulated by a shared operator is called an operon. The multiple genes…
Q: The lac operon is an inducible operon. What does this mean?
A: Operons are the groups of genes related to a single pathway and controlled by a single promoter.…
Q: DEFINE what is an Operon, and compare and contrast regulation of the LAC Operon and the TRP Operon.…
A: As per our honor code, we are allowed to answer one question at a moment. You have posted multiple…
Q: What is operon? How does the lac operon work?
A: A specific sequence of nucleotides in DNA is called DNA. It is a hereditary unit which is…
Q: A mutation occurs in the 5′ UTR of the trp operon that reduces the ability of region 2 to pair with…
A: In the absence of tryptophan, the trp operon would be expressed maximum as the trp repressor would…
Q: Explain what is Lactose operon and how does the system work .
A: The lactose operon also called as lac operon is a metabolism of lactose in E. coli in which operon…
Q: What is catabolic repression? How glucose concentration regulate catabolic repression of lac operon?
A: Genes are the functional segments of DNA. The cluster of these functional segments called genes is…
Q: What are differences between promoters and enhancers ?
A: Protein synthesis is the process by which genetic instructions stored in DNA (in regions known as…
Q: Why do scientists define the trp operon as repressible and the lac operon as inducible?
A: Operon is a combination of genes that are transcribed by the same promoter.
Q: What is the difference between the role of the operator and the role of the promoter in an operon
A: According to Jacob and monod concept, operon is a group of contiguous structural genes showing…
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- The lac operon consists of three structural genes, lacZ, lacY and lacA that are transcribed as a single polycistronic mRNA. You are given a new strain of Escherichia coli with the following lac operon genotype: p+0°Z•Y*A +// P*O*Z*Y+ A- (i) Explain how the lac I ³ gene affects gene expression.This figure represents the ABC operon, which is a negative inducible operon, and its associated regulatory gene. The structural genes produced by the operon convert molecule A into molecule D. When molecule A is present in the environment, it will bind to the regulatory protein, and operon expression will be induced. operator testR testR promoter regulatory protein testP testo structural genes test1 test1 test2 test2 test3 test3 structural proteinsThe lac operon consists of three structural genes, lacZ, lacY and lacA that are transcribed as a single polycistronic mRNA. You are given a new strain of Escherichia coli with the following lac operon genotype: p+0°Z•Y*A +// P*O*Z*Y+ A- (i) Explain how the lac I gene affects gene expression. (ii) Explain the function of the lacP in the bacterial operon. (iii) Which part of the lac operon is cis-dominant? Explain.
- Describe the 4 types of suppressor high copy suppression, bypass suppression, nonsense suppression, protein interaction . in your Own words. Often geneticists look for suppressors to find interactive proteins. Which of the type(s) of suppressors you put for part a will help to identify interacting proteins, and which type(s) will not? What are two (or one, if we don’t get a chance to talk about two of them in class) other techniques (not necessarily “genetic” techniques, but at least, lab techniques) that help to identify identifying proteins?O The lac operon in E.coli encodes enzymes necessary for the breakdown of lactose. For each enzyme (lac Z and lac Y), indicate with a + or-whether or not it is made when there is no lactose or when there is lactose. B-galactosidase (lac Z) No Lactose Permease (lac Y) Lactose Lactose No Lactose Genotype PP0 Z Y/I P*O*Z•Y* I'POCZ Y*/I P* O©Z*Y° P O Z'Y/I P'OʻZ'Y* PP O ZY*/IP*O*Z*Y* IP OCZ Y /I P*O*Z•Y* IPO ZY*/I* P*O©Z*Y• I'PO*Z Y*/IP'O*Z*Y°Given the following genotypes, explain, by answering the questions in each number, how the mutation (identified by a (-) superscript) will affect E. coli grown in lactose medium. Will there be a complete set ofgene products? (Yes/No) Will the lac operon be turnedon/off? Will the cell survive? (Yes/No) a. i + p + o + z - y + b. i + p - o + z + y + c. i + p + o - z + y +
- A number of mutations affect the expression of the lac operon in E. coli. The genotypes of several E. coli strains are shown below. ("+" indicates a wild-type gene with normal function and "-" indicates a loss-of-function allele.) Please predict which of the following strains would have the highest beta-galactosidase enzyme activity, when grown in the lactose medium. O CAP+ r* p* o* z O CAP* I P* o* z* O CAP* r* P O* z* O CAP I P* O z*The streptolysin S toxin made by S. pyogenes is encoded by a 9-gene operon, sagABCDEFGHI. Thinking about what a 3-line diagram would look like for this operon, answer the following questions. Write numeric answers only. For example, if your answer is 6 promoters, write only 6. 1) How many promoters control the expression of these genes? 2) How many locations does RNA Polymerase bind to get full expression of these genes? 3) How many ribosome binding sites are needed for full protein expression? 4) How many start codons will be needed for full protein expression? 5) How many mRNA strands will be produced with full operon expression? 6) How many proteins will be produced with full protein expression? 12) what is negative control of genes ? Does it need active form or inactive form of the repressor ? 3) What is role of RNA polymerase, operator and the repressor in repressible operon and inducible operons ? 4) How is eukaryotic gene expression different than bacterial gene expression ? 5) How does histone acetylation influence transcription ?
- In the presence of tryptophan, and a mutation in the allosteric domain that abolishes tryptophan binding______. a) the active repressor cannot bind trp), so operon gene transription is attenuated b) the inactive repressor cannot bind trpO, so operon gene transcription occurs] c) the active repressor binds trpP, so operon gene trasncription is repressed, the inducer cannot bind trpO, so gene transcription occurs d) the repressor binds to the corepressor, and an operon gene transcription occursFor each of the E. coli strains containing lac operon alleles listed, indicate whether the strain is inducible, constitutive, or unable to express beta-galactosidase and permease. (P+ and P- are functional and nonfunctional promoters, respectively) I+ P+ o+ Z- Y+ / I+ P+ oc Z+ Y+ I+ P+ o+ Z+ Y+ / I- P+ oc Z+ Y- I+ P+ o+ Z- Y+ / I- P+ oc Z+ Y- I- P- o+ Z+ Y- / I+ P+ oc Z- Y+ IS P+ o+ Z+ Y+ / I- P+ o+ Z+ Y-5 5 S 6 5 5 5 6 U 6 U 6 5:14 PM | 0.2KB/s HHHHH R R U RUUR ARU AP AP R U U R R AP R R R AP MOLECULAR...GENETICS. Describe gene regulation at transcription level. Explain the role of antsense RNA in control mechanism. Describe translational control mechanisms. Describe common DNA damages. Distinguish excision and mismatch repair. Describe the role of recA protein in recombination repair Elaborate on SOS repair mechanism. Define thymine dimer. How are they formed and repaired? Describe the molecular basis of mutation. 11 Leu+ Met+ Arg+ Write a detailed note on spontaneous mutation. Explain about mutant detection methods. Define reverse mutation. Describe the mechanism underlying Intragenic and intergenic suppressor mutations Describe the transposition mechanisms. 13 Vo LTE UNIT IV Time (Min) Describe the process of generalised transformation occurring in bacterial chromosome and plasmid. Elaborate on molecular mechanism and significance of transformation 22 Describe the process of…