67. What is the most accurate way to introduce mutations to particular regions in the genome?Using CrisprCas with particular RNA guides.Using restriction enzymes that perform staggered cutsUsing fluorescent probes with nuclease activity.Using engineered nucleases that have bigger palindromic sequences

An modification to the genome's nucleotide sequence of an organism, virus, extrachromosomal DNA, or…

What is the most accurate way to introduce mutations to particular regions in the genome? Using CrisprCas with particular RNA guides. OUsing restriction enzymes that perform staggered cuts Using fluorescent probes with nuclease activity. OUsing engineered nucleases that have bigger palindromic sequences

What is the most accurate way to introduce mutations to particular regions in the genome? Using CrisprCas with particular RNA guides. OUsing restriction enzymes that perform staggered cuts Using fluorescent probes with nuclease activity. OUsing engineered nucleases that have bigger palindromic sequences

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 10QP: Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands...

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Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.
Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymerase
When different individuals within a population have different numbers of restriction sites within the same region of DNA, what is this called? Do not just use an abbreviation.
An important feature of restriction enzymes is that each enzyme only recognizes a specific palindrome and cuts the DNA only at that specific sequence of bases. A palindromic sequence can be repeated a number of times on a strand of DNA, and the specific restriction enzyme will cut all those palindromes, no matter what species the DNA comes from. A linear DNA molecule is represented below. The DNA is represented by one line, although in actuality, DNA has two strands. If the DNA molecule has two restriction sites, specifically two repeats of a specific palindrome sequence, A and B, for a specific restriction enzyme: How many fragments would be produced if the DNA is cut by that enzyme? Number each fragment Which fragment would be the largest? Which fragment would be the smallest?
Which of the following most accurately describes the process of DNA cloning? set of laboratory procedures that consist of cutting a segment of DNA with restriction enzymes set of laboratory procedures that uses living cells to mass-produce specific DNA fragments set of laboratory procedures by which a DNA fragment is transferred from a living organism to a SNP chip the manipulation of DNA fragments in a laboratory using modern techniques of molecular biology set of laboratory procedures that consist of isolating of a DNA fragment from a living organism and inserting it into a plasmid
Which of the following is true about restriction endonucleases?a) Type I and II requires ATP to move along DNAb) Type I, II and III requires ATP to move along DNAc) Type II requires no ATP and cleaves DNA within recognition sequenced) Type II requires ATP and cleaves DNA within recognition sequence
Which of the following is NOT a tool used in recombinant DNA technology? Cutting specific DNA sequences using restriction enzymes. Adding Taq polymerase to copy DNA in PCR. Using primase to catalyze the formation of phosphodiester bonds. Inserting a gene of interest into a vector. Inserting a plasmid into bacteria to generate copies.
What ingredients are used in PCR? What role does each ingredient have in replicating DNA?
Describe the nature of recognition sites for restriction enzymes and the nature of the ends of the DNA that are left. Why do we need to run a gel electrophoresis after enzyme digestion
Is it necessary to cut the isolated genomic DNA of an organism with restriction enzymes for analyzing a particular gene sequence? Discuss.
Label the image below with ALL the pertinent information related to gene cloning. Make sure you use the following terms: bacterial plasmid, the gene of interest, recombinant plasmid, restriction enzymes, DNA ligase, insert plasmid into bacteria, cloning of plasmid in culture, etc.)
Which of the following would you need to clone a human gene? Choose all that apply A restriction enzyme Human DNA A donor goat nucleus An enucleated egg A surrogate mother DNA ligase A plasmid vector (or other type of DNA vector, e.g. phage, BAC)