Which activity would be considered transfection?O Inserting a DNA sequence from cows into a plant hostO Inserting a DNA sequence from humans into a bacterial hostO Using green fluorescent protein as a reporter geneO Inserting a DNA sequence from humans into a mouse hostO Using a gene that codes for antibiotic resistance as a reporter gene

Transfection It is defined as the process of introducing the naked or purified nucleic acids…

Which activity would be considered transfection? O Inserting a DNA sequence from cows into a plant host O Inserting a DNA sequence from humans into a bacterial host Using green fluorescent protein as a reporter gene Inserting a DNA sequence from humans into a mouse host O Using a gene that codes for antibiotic resistance as a reporter gene

Which activity would be considered transfection? O Inserting a DNA sequence from cows into a plant host O Inserting a DNA sequence from humans into a bacterial host Using green fluorescent protein as a reporter gene Inserting a DNA sequence from humans into a mouse host O Using a gene that codes for antibiotic resistance as a reporter gene

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter13: Early Life Forms And The Viruses

Section: Chapter Questions

Problem 6SQ

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Similar questions

How do some bacteria protect themselves against the viruses? protect themselves by making antibodies mutating the virus genome revers transcriptase assemble the capsid restriction enzyme
Which statement best explains the purpose of phage purification? To make sure that the phage sample only contains phages that are genetically identical to each other OtTo characterize phages based on plaque morphology To adapt the phage to lab conditions To determine which phage is the best at killing bacteria
The figure depicts five stages of the lytic cycle. ********* Select the statement that describes stage 3 of the lytic cycle, as shown in the diagram. The host cell synthesizes cellular proteins to prepare for lysis. The host cell incorporates phage DNA into its own genome. The host cell replicates the phage DNA. O The host cell synthesizes proteins that degrade phage DNA.
Categorize the following as being associated with transformation, conjugation, or transduction. Note that some items may be associated with more than one process. Transformation Conjugation Transduction requires a virus can transfer dead bacterium's DNA to a new host cell efficient way to get naked plasmid into E. coli cells in the lab involves a pilus describes type of horizontal gene transfer in bacteria cells need to be competent process exploitod by biotechnology transfer requires at least two living bacteria alters bacterial genome
Classify each statement as true of generalized transduction or specialized transduction. Generalized transduction Specialized transduction Answer Bank Genes transfer from random sites in the bacterial chromosome. This utilizes lysogenic phages. Genes that transfer depend on the prophage insertion site. This utilizes lytic phages. Phage inserts itself at a specific site in the bacterial chromosome. This requires accidental packaging of bacterial DNA in viral capsids.
phage can be "induced" as a result of DNA damage. Consequences of induction include. Temperate phage, e.g lambda phage; lytic cycle and transduction Lytic T4 phage, assembly, conjugation O Lytic phage, e.g. T4 phage; lysogenic cycle, prophage formation Temperate phage, e.g. lambada phage, prophage formation, transformation O T4 phage, lytic cycle, specialized trasnduction
What is the function of reverse transcriptase in retroviruses? converts host cell RNA into viral DNA translates viral RNA into proteins uses viral RNA as a template for DNA synthesis destroy the host cell's DNA uses viral RNA as a template for making complementary RNA strands
The clustered, regularly interspaced short palindromic repeats (CRISPR) system is like a bacterial adaptive immune system. After a bacteria is infected by a bacteriophage, CRISPR prevents the same type of phage from infecting the cell in the future by... integrating a phage DNA fragment into the chromosome, and transcribing it into a short RNA that binds a Cas nuclease to target and destroy invading phage DNA. modifying the attachment sites on the cell so the phage can't bind. O recognizing a molecule on the phage's surface and secreting a protein that prevents the phage from binding to the cell. recognizing a protein on the phage's surface and secreting an enzyme that destroys the phage.
Which of the following is NOT true about transduction? The bacteriophage's own viral DNA is the source of new genes for bacterial recombination Pieces of donor bacterial DNA can be inserted into the recipient bacterial cell's chromosome None of the other four answers (All are true statements about transduction) O Genetic recombination can result from bacteriophage (phage) virus infection of bacteria During the assembly of new phage particles, pieces of bacterial DNA are sometimes mistakenly inserted into new virus particles instead of phage (viral) DNA
What is the purpose of this structure at the end of the arrow that makes the bacteria more pathogenic? O used for cell reproduction O used for attachment and enter the host cell O used to prevent phagocytosis/ engulfment by immune cells O used to transfer plasmid DNA for antibiotic resistance between cells in conjugation
Which form of horizontal gene transfer is shown in the following diagram? Recipient Donor DNA transfer Donor Donor Pilus binding Pilus retraction Stabilization Segregation Transduction O Transformation O Conjugation MacBook Air DD
Lambda phage çan facilitate bacterial chemosomal DNA transfer through the process of specialized transduction / generalized transduction (copy and paste the correct answer) A by transferring specific bacterial DNA / bacterial DNA at random (copy and paste the correct answer) A from a donor cell / recipient cell (copy and paste the correct answer) A to a donor cell / recipient cell (copy and paste the correct answer).