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- You recently join a lab where professor is looking for photosystem of plants. He told you to isolate chloroplast and look for the photosystem II. Please select the correct sequence of events you are going to perform. options: Homogenize leaves à differential centrifugation à Fractional Solubilization à Affinity purification Homogenize leaves à Fractional Solubilization à differential centrifugation à Affinity purification Differential centrifugation à Homogenize leaves à Fractional Solubilization à Affinity purification Affinity purification à differential centrifugation à Fractional Solubilization à Homogenize leavesMelvin Calvin and Andrew Benson determined the steps in the light-independent reactions of photosynthesis by exposing the green alga Chlorella to CO2 labeled with the radioisotope carbon 14. By looking at which compounds the C14 ended up in, they were able to identify all intermediates in this cyclic pathway. Why did they predict correctly that the same set of intermediates would be formed in the light reaction in land plants?You receive kinetic data for a transferase reaction where AX and B are substrates, while A and BX are products. You are asked to establish the reaction mechanism. Which graph will you draw first? A,B,C or D A. 1/Vo against [AXo] at different concentrations of [BXo] B. 1/[AXo] against 1/[Bo] at different concentrations of [BXo] C. 1/Vo against 1/[AXo] at different concentrations of [Bo] / D. 1/Vo against 1/[AXo] at different concentrations of [1/BXo]
- To assess photosynthesis, we relied heavily on a spectrophotometer to measure the color intensity of DCPIP in our solution. We set the spectrophotometer at wavelengths in the visible spectrum. What is the approximate wavelength range (in nm) for the visible spectrum? For example, the ultraviolet spectrum wavelength range is approximately 10-400 nmSolar-induced fluorescence can be used to infer net photosynthesis. This is because this solar-induced fluorescence is able to measure the light that is used by the chlorophyll-a pigment (650-850nm). There is a linear relationship between this solar-induced fluorescence and the gross primary production of a plant. How does measuring chlorophyll-a pigment with solar-induced fluorescence help us infer net photosynthesis?Assume that two pigments, red and bluc, mix to give the normal purple color of petunia petals. Separate biochemical pathways synthesize the two pigments, as shown in pathways I and Il in the accompanying diagram. "White" refers to compounds that are not pigments (total lack of pigment results in a white petal). Red pigment forms from a yellow intermediate that is normally at a concentration loo kow to color petals. Bluc mixed with yellow makes green. Assume that no mutations are lethal. Pathway I.. White Blue Pathway I White, Yellow Red Pathway II White, White, A third pathway, whose compoundk do not contribute pelal pigmentation, normally docs not allfect the bluc and red pathways. However, if one of its intermediates (white3) should build up in concentration, it can be converted into the ycllow intermediate of the red pathway. In the diagram, the letiers A through E represent enzymes. The enzymes' correspoonding genes, all of which are unlinked, may be symbolized by the same letters.…
- You performed an experiment on photosynthesis on Elodea, using bromothymol blue as an indicator forpH. Your two light conditions were red light and blue light. You found that in blue light, your solutionturned a nice cobalt blue, and in red light it turned green.a. What can you conclude about the efficiency of photosynthesis in Elodea regarding those twowavelengths?b. What would your conclusion be if your negative control (no Elodea in white light) turned greenalso?Bacterium Y is photosynthetic. You want to decrease the ability of Bacterium Y to produce glucose through photosynthesis. Explain how you could manipulate the light reactions of photosynthesis to decrease glucose production during the dark reactions in Bacterium Y. Include the name of the pathway that makes glucose during the dark reactions, which/how molecules from the light reactions are used in this pathway, and what specific steps of this pathway would be affected by your manipulations.a.com/assignments/viewer/classes/329ea460c4114349972e507a776991d0/assignments/b729d9ce127845af8a13a22bc6c88f24/contents/...0* Study Plan Granada G GOOGLE Photosynthesis synthesis 1. Describe the overall changes in matter that occur during the light- independent reactions. What does the plant start with and what does it end with? What is the source of energy that powers this change? Type your answer here. oficinavirtual Data Parameters.xlsx https://escuelaposg... From 6 Photosynthesis-E....pptx 6 ADP M ATP Light-Dependent Reactions UGR ES ACCOUNT PDF 00000 10 000 Light-Independent Reactions 60 CO2 2000 POST The Calvin Cycle-R.....pdf Connected A 12 000 STROMA 12 M 000 12 ATP https://webmailest..... 12 ADP 12 Sugars and Other Compounds 12 NADP+ NADPH CYTOPLASM https://biblioteca From Light- Dependent Reactions The Process of Ph.....pptx The Process
- Differentiate non-cyclic light reaction and cyclic light reaction1.) True or False. In the second step of Light Dependent Reaction, P680 loses its electron and becomes positively charged (P680+)An experiment was done to determine how color of light and distance affects photosynthetic activity of the pond weed. Pond weed was placed in a test tube containing a solution of sodium hydrogen carbonate, and had its end cut; light was placed 50 cm away and four colors were tested (clear, red, blue, and green). After placing the light, the pondweed was given a certain amount of time to adjust before counting the bubbles, bubbles were counted for 1, 3, and 5 minutes. Only the bubbles that came out of the cut end was counted to represent the rate of photosynthesis. Below is the tabulated results. What are the possible factors or errors that could have affected clear/white light to have lower photosynthetic activity? In theory this should have the highest rate.