You have cloned the gene for a human erythrocyte protein, which you suspect is a membrane protein. You deduce the amino acid sequence of the protein from the nucleotide sequence of the gene. From the amino acid sequence alone, you evaluate the possibility that the protein is an integral protein. How could you determine whether the protein is an integral protein? Use a hydropathy plot. If the plot shows a hydrophobic region of 20 or more residues, it is likely an integral protein. Use a hydropathy plot. If the plot shows a hydrophilic region of 20 or more residues, it is likely an integral protein. Determine the distribution of proteins. If its distribution is asymmetric, it is likely an integral protein. Determine the distribution of proteins. If its distribution is symmetric, it is likely an integral protein. Suppose the protein proves to be an integral protein with one transmembrane segment. How could you determine whether the protein is oriented with the amino terminus on the outside or the inside of the cell? Treat protein reacts, the amino terminus is with a membrane-impermeant reagent known to react with primary amines. If the of the erythrocyte membrane.

You have cloned the gene for a human erythrocyte protein, which you suspect is a membrane protein. You deduce the amino acid sequence of the protein from the nucleotide sequence of the gene. From the amino acid sequence alone, you evaluate the possibility that the protein is an integral protein. How could you determine whether the protein is an integral protein? Use a hydropathy plot. If the plot shows a hydrophobic region of 20 or more residues, it is likely an integral protein. Use a hydropathy plot. If the plot shows a hydrophilic region of 20 or more residues, it is likely an integral protein. Determine the distribution of proteins. If its distribution is asymmetric, it is likely an integral protein. Determine the distribution of proteins. If its distribution is symmetric, it is likely an integral protein. Suppose the protein proves to be an integral protein with one transmembrane segment. How could you determine whether the protein is oriented with the amino terminus on the outside or the inside of the cell? Treat protein reacts, the amino terminus is with a membrane-impermeant reagent known to react with primary amines. If the of the erythrocyte membrane.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter11: Genome Alterations: Mutation And Epigenetics

Section: Chapter Questions

Problem 10QP: If the coding region of a gene (the exons) contains 2,100 base pairs of DNA, would a missense...

See similar textbooks

Similar questions

Discuss the following statement: “from the nucleotide sequence of a cDNA clone, the complete amino acid sequence of a protein can be deduced by applying the genetic code. thus, protein biochemistry has become superfluous because there is nothing more that can be learned by studying the protein.”
When a protein was denatured and lost its function due to its spatial structures were disrupted. Which of the following was disrupted during the denaturing process?
You are a molecular biologist studying a defective human protein that is known to be a cause of a rare cancer. You isolate the suspected protein from a person with this cancer and compare it to the same protein in a person without the cancer. You discover that the protein isolated from the cancer patient is slightly smaller in size when compared to the protein from the unaffected patient. Furthermore, you discover that the protein from the cancer patient differs in almost every amino acid starting after the 23rd amino acid. What is the likely explanation?
I are studying pancreatic islet cells and have isolated, cloned, and sequenced a novel protein that you postulate has 4 transmembrane segments. Explain why the sequence would lead to this hypothesis (what procedure would I have applied).
The CFTR membrane protein has an alpha helix that spans the membrane. The amino acid sequence of the central portion of this alpha helix, which is embedded within the membrane, is which one of the sequences below? Sequences are provided in the single letter code. a. VVVEVVVVEVVEEEVE b. VVVLVVVVLVVLLLVL c. VVVLVVVVREERRRER d. EEEREEEEREERRRER
You are characterizing a DNA-binding protein, and have used genetic experiments to identify a domain required for the interaction of the protein with DNA. Your secondary structure analysis software predicts that the domain forms a β helix, but you are unconvinced. What experiments could you perform to confirm the presence of a β helix in this region?
a molecular geneticist hopes to find a gene in human liver cells that codes for an important blood-clotting protein. he knows that the nucleotide sequence of a small part of the gene is gtggactgaca. briefly explain how to obtain the desired gene answer
I am studying pancreatic islet cells and have isolated, cloned, and sequenced a novel protein that you postulate has 4 transmembrane segments. What kind of experiment would allow me to test this hypothesis that there are 4 trans membrane segments?
A scientist wants to use a technique that allows to shuffle protein domains. Protein A and Protein B are 5 and 7 different domains respectively. If 3 domains from A and 4 domains from B are chosen and paired how many primers are needed to amplify domain sequences to prepare all constructs?
What is the difference between an autonomous and a nonautonomoustransposable element? Is it possible for nonautonomous TEs to move? If yes, explain how.
In a particular region of the genome of a certain bacterium, one DNA strand is transcribed to give rise to an mRNA for protein A and the other DNA strand is transcribed to give rise to an mRNA for protein B. a) Would there be any problem in expressing the two genes? 2. b) If a mutation occurred to affect the structure of protein A, what would you observe in the structure of protein B?
Which of the following describe how the order of nucleotide bases along a gene in the DNA ultimately determines the primary structure of a protein and how the primary structure ultimately determines the three-dimensional shape and function of the protein coded for by that gene. A Certain amino acids in a protein interact with other amino acids in that same protein and so the order of amino acids ultimately determines the 3-dimensional tertiary structure of that protein. B The order of nucleotide bases along the DNA is transcribed into complementary tRNA which is translated into the correct amino acid sequence for the protein by mRNA. C The order of nucleotide bases along a gene determines the order of amino acids in the resulting protein. The order of amino acids in the protein is called its secondary structure.