6. Connection to Biology. Draw the mechanism of dephosphorylation by a phosphatase that involves a two-step mechanism (i.e., indirect hydrolysis). Three boxes (one drawn to get you started) have been provided for you to draw each step in the mechanism. Make sure to draw curved arrows (including on the starting diagram provided) indicating the movement of electrons and show formal charges. Hint: The cysteine residue on the enzyme is involved in the mechanism. Hint: Try to account for all of the proton movement (H+) as well, which is not explicitly described in your textbook for this reaction, but you can do it! Some water and a proton have been provided to guide you. H Step 1 HN Phosphatase Enzyme Protein Substrate H. H H+ Step 2

6. Connection to Biology. Draw the mechanism of dephosphorylation by a phosphatase that involves a two-step mechanism (i.e., indirect hydrolysis). Three boxes (one drawn to get you started) have been provided for you to draw each step in the mechanism. Make sure to draw curved arrows (including on the starting diagram provided) indicating the movement of electrons and show formal charges. Hint: The cysteine residue on the enzyme is involved in the mechanism. Hint: Try to account for all of the proton movement (H+) as well, which is not explicitly described in your textbook for this reaction, but you can do it! Some water and a proton have been provided to guide you. H Step 1 HN Phosphatase Enzyme Protein Substrate H. H H+ Step 2

Organic Chemistry

9th Edition

ISBN:9781305080485

Author:John E. McMurry

Publisher:John E. McMurry

Chapter21: Carboxylic Acid Derivatives: Nucleophilic Acyl Substitution Reactions

Section21.SE: Something Extra

Problem 42MP

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