The time taken to complete the transcription process of RNA in E. coli is to be calculated.

An amino acid is coded by 3 nucleotides called as codon. The maximum rate of transcription of RNA in E. coli is 80 bp/s approximately and the minimum rate for the same is approximately 40 bp/s.

Given:

Molecular weight of the protein= 74kDa = 74000 Da

The average molecular mass of an amino acid = 110 Da.

Therefore, the number of amino acids present in 74 kDa protein is calculated as follows:

We know, 1 amino acid is coded by 3 nucleotides.

Thus, the number of nucleotides in 74 kDa protein will be:

We know the maximum rate of transcription of RNA in E. coli is 80 bp/s.

The total time needed for transcription is calculated using the formula given below:

We know the minimum rate of transcription of RNA in E. coli is 40 bp/s.

The total time needed for transcription is calculated using the formula given below:

Thus, the transcription process of RNA in E. coli, when maximum and minimum rates of transcription are considered, takes 25 s and 50 s respectively.