In this reaction, DCPIP (2,6-dichlorophenolindophenol) will react with Vitamin C by redox titration (turning the solution from pink to colourless)(en.wikipedia.org). Vitamin C and DCPIP react in a 1:1 mole ratio and hence if a known amount of DCPIP is used; it is easy to find the mass of ascorbic acid sample as it is a direct measure (saps.org.uk)(microbiologyinfo.com). HC12H6Cl2O2N + 2H+ + 2e- HCl12H8Cl2O2N Red
in neural tissue, while the endotherial isoform is found in the endotherial tissues. The inducible isoform, iNOS, is found throughout the body and produces nitric oxide in response to infection after binding to calmodulin (1). All isoforms convert arginine to nitric oxide and releases citrulline. (2) Many cofactors, including NADPH, FAD, FMN, heme, and O2¬ are needed for this conversion. (2) The inducible isoform produces the most nitric oxide and for the longest amount of time (3) Nitric oxide synthase
REACTION PAPER ON HERPES Sexually transmitted diseases or STD's are infectious diseases that can be Spread by sexual contact. Some can also be transmitted by nonsexual means, But these make up a majority of the total number of cases. An estimated 10 to 12 million Americans have sexually transmitted diseases, several kinds of Which are epidemic, including
Cancer therapy has revolutionized from conventional nonspecific approaches such as surgery, chemotherapy and radiotherapy to a specific method, immunotherapy. Clinical data and results illustrate significant increase in demand for strategies to improve the health of cancer patients. improve immunotherapy during the past two decades because of its major advantages compared to conventional therapies [advantages of immunotherapy to conventional]. Immunotherapy works by integrating immune system to fight
can cause an Allergic Reaction. These reactions can be caused by certain species of shellfish or all shellfish, you can be allergic to fish and not shellfish or vice versa. In the following pages I will cover shellfish allergies, shellfish species, the types of reactions and the treatment options as well as coping with the allergy. What is a Shellfish Allergy? A shellfish allergy is an allergic reaction caused by contact with or consumption of shellfish. An allergic reaction to shellfish can range
Beta-Galactosidase and Western Blot 0 1. EMG 9 and EMG 26 contain strain _lac-_(I- Z+ Y+) and strain _lac -_ (I+ Z- Y-)respectively.Three genes huddled together on the chromosome are required for two strains of _E.coli_ to utilize lactose.Consisting of three genes, namely, _lacZ_, _lacY_ and _lacA_, the _lac_ operon orderly handles these genes to code specific enzymes necessary for the metabolism of lactose. The genes _lacZ_, _lacY_ and _lacI_ would code for beta-galactosidase, galactosidase permease
The non-enzymatic reaction of reducing sugars with amino groups of amino acids, peptides and proteins is called as non-enzymatic glycosylation or simply glycation. This non-enzymatic reaction ultimately results in the formation of complex brown pigments and protein-protein crosslinks that were first studied under defined conditions by Maillard in the early 1900s (Maillard and Gautier, 1912) and the reaction came to known as the Maillard reaction. There is a condensation reaction between reducing sugar
functions. Of all types of collagen, type I collagen is the most abundant form and is responsible for substituting 90% of the body’s collagen. In view of the important of collagen type I collagen in human body structures and functions, the aim of this paper is to provide in-depth information about type I collagen post-translational modification process, and diseases that are governed by it. Collagen has few subgroups and fibril forming collagen is the largest group in the human body. Type I collagen
phosphorylation was first introduced by Edmond Fischer and Edwin Krebs in the year 1955, where they elucidated the necessity of ATP and a kinase (Known then as “converting enzyme”). Interestingly, a reaction which involved protein phosphatases (PP) was reported a decade earlier, but it was not characterized as PP reaction because of the inability to detect inorganic phosphate as a product.1 Since the above mentioned early discoveries, it has been well established in eukaryotic cells that reversible phosphorylation
one of the most versatile molecules in the living cell and it can perform various diverse functions, such as regulation of genetic information, gene expression, synthesis and modification of proteins, mediation of enzymatic activity of biological reactions and others. All of these essential functions for cellular biological processes are accomplished when RNAs interact with special type of proteins called RNA binding proteins (RBPs). So, why do we need to study RNA-protein interactions? The reason