Molecular biology

beads to remove any unwanted ligated products. The universal and indexed sequences were amplified by PCR using adaptor-ligated DNA fragments, NEBNext High Fidelity 2X PCR Master Mix, index primers provided in NEBNext Multiplex Oligos for Illumina, and Universal PCR Primers provided in NEBNext Multiplex Oligos for Illumina. Once the PCR was done, the adaptor-ligated DNA was cleaned up using AMPure XP beads to remove any unwanted products. Figure 3: Pre-Sequencing Sample Preparation. Samples were

In the current study domain III of PE was deleted and replaced by STXA, by the recombinant DNA technology. STXA was transferred across the cell membrane using domain IIof PE. This domain facilitates the internalization of extracellular compounds into the cytosol, and can translocate heterologous molecules into the target cells(15). Then PE II and STX are connected to fragment variable (Fv) domains of herceptin and create a (single chain) scFv immunotoxin(16). Material and Method 1.1. Material and

File #1 (Hair) Link: http://www.exploreforensics.co.uk/forensic-cases-murder-leanne-tiernan.html Suspect(s): John Taylor Victim(s): Leanne Tiernan How hair was used to solve the case: In Tiernan’s scarf, many hairs were found. although having insufficient root DNA, “The scientists found very small amounts of DNA in the hair shaft and used mitochondrial DNA testing to match it to John Taylor.” File #2 (Hair) Link: http://losangeles.cbslocal.com/2010/08/31/1977-cold-case-murder-solved-by-a-hair/amp/

(SUNO) may change that trend, thanks to its infant Forensic Science program and its recently completed Forensic Science Laboratory. The new program and lab are attracting more students to the university and are igniting a love for science. image1 Biology professor Dr. Christian Clement and student Diaminika Moss tour the new SUNO Forensic Science Lab. (Credit: SUNO) SUNO is the first university to offer an undergraduate Forensic Science degree program in the state of Louisiana, and one of only four

The researchers were tasked to investigate phenotypic changes in Paramecia. Paramecia are single celled organisms that have appendages attached to their bodies called cilia. The cilia help by allowing a Paramecium to move away from toxins, and towards a food source. The cilia are important because their water environment lacks movement. In the lab, the Paramecia were grown in flask that contained the wheat media, and the bacteria, Klebsilla pneunomiae, was inoculated into the flask. The wheat media

Witkop syndrome is manifested by defects in the nail plates of fingers and toes typically and hypodontia, with normal hair and sweat gland function. There is a pattern of missing teeth.8 Absent maxillary incisors, second molars, and maxillary canines are the most common missing teeth.9 Genetically, mutations in genes MSX-1, PAX9, PsITX2, LTBP3, WNT10A, EDA and EDARADD, AXIN2, and IKBKG have been found associated with oligodontia. Mutations in MSX-1 have been shown to be associated with Witkop’s

2.3 Exposure experiment in Daphnia magna The effects of MP exposure on life history parameters were tested in a 21-d reproduction test according to OECD guidelines at one concentration of each particles size: 1000 particles/L (0.1,1,10 µm), subjected to the environment concentration about 1.1 items/mL has been reported(Moore et al., 2011). In brief, there were triplicates for each control and treatment group an experimental series. Five neonates (< 24h) were incubated respectively in 50 mL medium

The purpose of this experiment was to determine how different surface temperatures affect the appearance of latent finger prints. In order to run this experiment, thirty Standard Select Carolina Microscope Slides were wiped with rinsed with tap water then blotted dry with Kimwipes. Ten microscope slides were chilled to negative nineteen degrees Celsius, ten others were left out, resulting in a temperature of twenty-three degrees Celsius, and another ten were incubated to thirty-seven degrees Celsius

Histochemistry studies can reveal the molecular interactions of structures, such as neurotransmitter production and activity, metabolism, immune function, and other interactions. Histochemistry can also be used to reveal chemoarchitecture or chemical neuroanatomy. Immunocytochemistry is a type of histochemistry that makes use of antibodies to visualize cell types, axons, neuron structures, glial processes, blood vessels, or molecular components of cells like neurotransmitters and intranuclear

elongation (essentially, DNA replication). Once this is complete, it is common to use agarose gel electrophoresis and UV light to visualize the bands of separated DNA. In terms of this experiment, cheek cells were obtained from UNR students in the Biology 395 lab. Using the two described techniques, genotypic frequencies were calculated for the human-specific Alu insertion on chromosome 16: homozygous for the insertion (+/+) heterozygous for the insertion (+/-), and homozygous for the absence of insertion