(isoelectric focusing) was performed using the PROTEAN IEF cell (BioRad, USA) for a total of 60 kVh at 20ºC. IPG strips were equilibrated for 20 min in the equilibration solution (50 mM Tris–HCl pH 8.8, 6 M urea, 20 % glycerol, 2 % SDS, 0.01 % bromophenol blue) containing 2 % DTT and then alkylated for 20 min in the equilibration solution containing 2.5 % iodoacetamide. For second-dimension electrophoresis, the strips were transferred on top of gradient SDS-polyacrylamide gels (18 cm×20 cm×1.0 mm 8%-16
The natural rubber industry products have a large demand globally for their various uses in different applications. Rubber is used to manufacture products in every field, from automobile parts, medical devices, plumbing, electrical, stationary, etc. India is ranked third in the world producers of natural rubber [4]. In the Dakshina Kannada region of Karnataka agriculture is one of the main occupations of the locals. Rubber, Arecanut, Paddy, Coconut, etc., are commonly grown in this region of which
Introduction Malignant tumors have become a major problem in public health. Chemotherapy, radiotherapy, and surgery have been the backbone of current treatment for many types of human cancer. Despite providing variably effective treatments in many cases, they are still hindered by the risk of cancer recurrence due to acquired chemo- or radio-resistance properties of some tumor cells especially tumor initiating cells [1, 2]. Recent advances in our understanding of immune system and its role in attacking
c CHAPTER - 1 INTRODUCTION Parle Biscuits Ltd was set up in Bombay in year 1932. It expanded with high frequency in different parts of India. In this process of expansion the unit at Bahadurgarh (Haryana) was established in 1981 as Parle Biscuits Ltd. The plant is one of the most mechanized and advanced in the country. The location of the factory is well suited, being at national highway near Delhi. It greatly facilitates the delivery of raw materials and dispatch of finished products
different factors: ¡¤ changing the surface area of any solids involved ¡¤ changing the concentration of reactants in solution ¡¤ changing the temperature at which it is carried out ¡¤ the use of a catalyst I am going to investigate into exactly how one of these variables affects the reaction rate and I believe the most suitable variable to change is the concentration of the solution as all
Executive Summary On December 3, 1984, toxic poisonous methyl isocyanate gas leaked from Union Carbide India Limited (UCIL 's) pesticide plant in Bhopal. The gas leak triggered a disaster that is now widely recognized as the world worst industrial catastrophe. Thousands of people were killed instantly and more than 25,000 people have died of gas-related illnesses, several thousands more maimed for life since. Union Carbide negotiated a settlement with the Indian Government in 1989 for $470 million