6.71 mL of 4-methylcyclohexanol, 2.0 mL of phosphoric acid and 30 drops of concentrated sulfuric acid were placed in a round bottom flask. The liquids were then mixed using a glass stirring rod after which a boiling stone was added. Mixture was then distilled using a 25-mL receiver flask sitting in an ice bath. Heating of mixture was regulated to ensure that reaction and isolation of the starting material were fully completed, the process was carried out until there was no liquid remained to be
The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in
The purpose of this experiment was to determine the relationship between tail spine length and hemoglobin levels as well as the relationship between tail spine length and heart rate. The concentration of the hemoglobin in Daphnia is dependent on the oxygen available to them.
1.5mL of phosphoric acid including 3-4 boiling chips were also added to the 25mL flask. The short path distillation apparatus was set up as shown in Figure 1. A heating mantle was used to heat up the 25mL flask. The solution was distilled to the receiving flask until a small amount of liquid remained in the initial RBF flask. At this point the presence of thick grey smoke pulling over into the entire apparatus was observed. The apparatus was then left to cool down. Through the use of pasture pipette, the aqueous layer from the distilled solution was drawn out. Sodium carbonate was then added to the remaining organic solution in order to check the pH and to verify the basicity of the solution. The aqueous layer was again drawn out from the solution. Next, 0.5g of sodium sulfate was added to the remaining organic layer and was swirled until the liquid appeared to be dry and clear. The alkenes were transferred into a clean 10mL flaks using another clean pasture pipe. The apparatus from the first distillation was rinsed off with
The purpose of this lab was to identify unknown bacteria cultures using various differential tests, and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were Gram stain, Catalase, Mannitol Salt Agar (MSA), Blood Agar, Novobiocin, Coagulase, and DNAse (Alachi, 2007).
Discussion As part of the experiment, the percent composition of each component of the mixture was calculated. 51% of the components were retrieved from filtration while 49% of the solvents were retrieved from dissolving the components in a solvent. The original mixture was one globular solid-like structure.
How should the unknown microscopic organisms be classified? The concept of the experiment was to conduct a systematic observation about the traits of unknown organisms, and classify them to the right group. Making detailed observations about the traits, made it possible to identify which cell the trait belonged to because different cells have different traits. Animal cells do not have a cell wall, and has one or more small vacuoles. Plant cells has a cell wall, rectangular (fixed shape), and has a chloroplasts. Cytoplasm, Endoplasmic Reticulum, Ribosomes, Mitochondria, and Golgi apparatus to name a few.
2. (5 pts) List and explain the names and affiliations of the various characters/stakeholders in this story – I’m looking for us to use the story to map out the complexities that are generally associated with solving public health puzzles – the stakeholders you list and explain here should apply to many of the cases we consider going forward.
In this experiment, 1,2-cyclohexene was taken and made it 1,2-cyclohexanediol in two separate reactions. Two products were formed in each respective reaction: cis-1, 2-cyclohexene and trans-1, 2-cyclohexanediol. To figure out which product was formed in each reaction, TLC was used to test the stereochemistry from the products compared to the actual compounds. Each retention factor was compared to the actual product retention factors to see if the reactions had been done properly.
Since the purpose of our experiment was to synthesize Tetraphenylcyclopentadienone from benzyl,dibenzyl ketone,absolute ethanol ,and potassium hydroxyde, we finally got our product at the end which mean we were able to accomplish our purpose. that product is very dangerous for human and very toxics for aquatic organisms.
The investigation is showing how enzymes work inside a mammal's stomach. Rennin is the enzyme found in young mammals and has more effect
The mean voltage of the battery terminals while connected to the identification resistors is presented in Figure 4 12. These samples have been pulled out from the voltage sensor of the PEB panel. The voltage decreased as expected from 12.53 to 12.5 during first 20 seconds of connection to the
(E)-3-benzylidenepiperidin-2-one (7a) A solution of (1E,2E)-2-benzylidenecyclopentanone O-tosyl oxime (6a, 0.500 g, 0.00146 mole) and SOCl2 (0.521 mL) in dry dioxane (30 mL) was stirred with the aid of a magnetic stirrer at room temperature for 10h. Thereafter, water was added to the reaction mixture and it was extracted with diethyl ether (3×20 mL). The combined extracts were washed with water, NaHCO3, and water and dried over anhydrous Na2SO4. Rotaevaporation of the solvent furnished a solid which upon crystallization from benzene furnished (E)-3-benzylidenepiperidin-2-one (7a) as light brown crystals, yield 45%, mp 126–128°C
Concentration response curves to methacholine showed leftward shift in asthmatic group compared to group C, but the curves of treated groups with dexamethasone and all concentrations of curcumin were shifted to right compared to group A (Fig. 4a). The EC50 value in group A was significantly lower than group C (p<0.001, Fig. 4b). The values of EC50 in treated groups with all concentrations of curcumin and dexamethasone were significantly improved compared to the group A (p<0.001 for all cases, Fig. 4b).
After the mixture finished refluxing, the flask was then cooled on ice. A sulfuric acid solution was then prepared by pouring 4.5 mL of concentrated H2SO4 over 50 grams of ice and then diluted to 75 mL by adding enough tap water to reach 75 mL. The sulfuric acid solution was then cooled on ice.
Preparation of 3,3,5-trimethylcyclohexanol mixture: Isopropanol (12.5 mL, 163 mmol) and a KOH pellet were added to NaBH4 (17.8 mmol, 673mg) before 3,3,5 trimethylcyclohexanone (20 mmol, 3.16 mL) was added to the suspension. The reaction mixture was stirred (30 minutes, room temp.) and then added to brine (10.0 mL) in a separatory funnel. The product was extracted from the aqueous layer using 10% hexane (3 x10mL) before the combined organic layers were washed with brine (10.0 mL), dried with Na2SO4, and concentrated. The crude product was distilled using a vacuum aspirator to produce a clear, colorless liquid containing cis-3,3,5-trimethylcyclohexanol (1) and trans-3,3,5-trimethylcyclohexanol (2) (BP: ~105°C, 977mg, 38.6% yield), characterized