A recent public health concern has implicated polyethylene terephthalate (PET) plastics, commonly used in recyclable plastic water bottles, as a common source of carcinogenic xenoestrogens. Xenoestrogens act as hormone disruptors, and can alter the natural homeostasis of the human body. Exposure to endocrine disrupting chemicals has been known to cause cancer, sexual dysfunction, and problems during puberty. Better understanding exposure to these chemicals will help improve awareness of the issue and hopefully prevent some exposures. An experiment has been devised to study the quantity of xenoestrogens present in two common types of water bottles, BPA free bottles and recyclable single-use plastic water bottles. Due to the…show more content… Our group of three scientists has access to Kettering University’s Applied Biology laboratories equipped with the necessary instrumentation to perform the biological assays needed to test our hypothesis. The team’s combined experience and background in microbiology, chemistry, and biology will play a key role in the process. Between us, we have participated in research through cooperative education and on campus opportunities, as well as completed several upper-level biology and chemistry courses, including Analytical Chemistry, Biochemistry, Molecular/Cellular Biology and Biological Techniques.
Specific Aim 1: Quantify Any Xenoestrogens Present at Ambient Temperature (20°C/68°F)
A yeast bioassay will be employed to determine what quantity, if any, of xenoestrogens are present in the water samples taken from a single-use, plastic water bottle and a BPA-free water bottle, both incubated for 24 hours at ambient temperature (20°C/68°F). The assay utilizes relatively inexpensive and easy to maintain yeast (Saccharomyces cerevisiae). The yeast used will be MATa leu2-112 ura3-1 trp1-1 his3-11, 15 ade2-1 can1-100GAL SUC2 which was cotransformed with the DSY219 plasmid containing: pSO4-123 (ER/TRP) for human estrogen receptor alpha and pSO4-124 (ERE-lacZ/URA) for chemiluminescence (response). The response will be measured with a standard microplate microluminator for each sample against a