Caffeine Reasearch Lab Report

Many manufactures release the caffeine content of their products publically, but not always, and new products and flavors are continuously introduced to the market. If quality checks are not performed, manufactures may alter the caffeine and benzoic acid content to suit the demands without public knowledge. To ensure the levels of caffeine and benzoic acid in products do not exceed the established safe limits and to inform the public of the amount of these compounds being consumed, various methods of analysis have been performed. Before the introduction of modern techniques, spectrophotometric methods alone were used to determine concentration of a compound in a mixture.6 The caffeine content in coffee, tea, soft drink, and energy drinks were determined using an immunoassay.7 The caffeine content in mixtures also used to have to be extracted before quantification.8

Both decaffeinated and caffeinated coffee and tea can cause gastrointestinal problems in certain individuals as they are both acidic in nature. People don’t realize that decaffeinated does not mean that the beverage does not contain caffeine; it just means that there is less of it in that beverage. I question a study that Malcolm Gladwell describes in “Java Man” in which King Gustove III ordered an experiment to show the harmful effects of caffeine. “A convicted murderer was sentenced to drink cup after cup of coffee until he died, with another murderer sentenced to a lifetime of tea drinking, as a control” (Gladwell, 233). Interestingly, in the experiment, the subjects outlived King Gustov III and the doctors performing the experiment. The study does not tell us of any heath issues that the subjects

← I would repeat the experiment with the caffeine solution more times, so that the results I would get will be more

III. “The U.S. Food and Drug Administration (FDA) and the American Medical Association (AMA) have estimated that the average daily caffeine

1. To assess the effect of caffeine on persistence researchers assign their participants to a group that gets a non-caffeinated beverage or a group with a caffeinated beverage. They measure how much time the participants spend on a difficult puzzle.

The effects of caffeine and alcohol on daphnia are expressive of whether these substances are harmful or beneficial to the organism. By understanding the results of this experiment, it may also be understood how these substances effect humans. In this study, one daphnia was exposed to increasing levels of alcohol, while the other was exposed to increasing levels of caffeine, each in order to test the hypothesis that when given amounts of caffeine and alcohol, the daphnia will be affected the same way a human would. The effect of each substance was measured by the daphnia’s heart rate one minute after the substance was added. Results reveal that alcohol slows the heart rate, while caffeine increases heart rate. Furthermore, caffeine shows a

B. Thesis statement – Caffeine can have many different effects on the body depending on the amount of consumption.

An independent samples t-test was conducted to examine the difference between experimental conditions on test performance. The results indicated a significant difference between participants who consumed the caffeinated beverage and participants who did not, with participants in the caffeinated group (M = 7.64, SD = 2.41) performing worse than participants in the non-caffeinated group (M = 9.81, SD = 3.16), t (97) = 2.14, p < .05.

Components containing caffeine were composed into stock solutions. These solutions were diluted to 1: 10 substance: mobile phase. A stock solution of caffeine was diluted 1:50. A sequence of diluted caffeine solutions were prepared for use as a standard (ppm): 1, 2, 4, and 10. Solutions of acetaminophen, acetylsalicylic acid, and Goody’s Powder were developed to differentiate chromatographic peaks observed. These solutions were subjected to HPLC for examination of the observed peak area and retention time for the set of compounds. Comparison of retention time allowed for the differentiation of peaks observed. The peak area obtained was utilized to determine the relative concentration of caffeine present in Goody’s Powder based on the relationship obtained in the standard. The content of caffeine present in Goody’s Powder by percent weight was identified.

The CARROT test showed that people who consumed and believed they have consumed caffeine was significantly faster in sorting cards compared to the other groups in rewarded trial. In case of the non-rewarded trial there was a significant difference between the mean of rate of sorting between each group regardless of their drink type or

Thesis Statement: Caffeine is something that almost everyone has consumed and some people are even addicted to, it has negative and positive effects on one’s health.

In this project the effects that different caffeine levels have on human urinary pH have been tested. The hypothesis, If the amount of caffeine in beverages increases then the pH of urine will decrease when the pH of urine is a function of caffeine levels, was the basis of this experiment. Three test subjects consumed three different caffeinated beverages, each with different caffeine levels, including Monster Energy Zero Ultra, Dunkin’ Donuts Hazelnut Iced Coffee, and Diet Dr. Pepper. The Monster had a caffeine level of 70 mg per 8 fluid ounce serving. The Diet Dr. Pepper had the lowest caffeine content with 60 mg per 20 fluid ounce serving. The iced coffee had the highest caffeine level with 279 mg per 24 fluid ounce serving. Test subjects

They performed the experiment in 20 days, which divided into 2 sections of aspartame test and placebo test with each period last for a week and before each period begins, there will be a 3-day “washout” period to make sure there is no reaction from previous section test. Before the first section, all participants were asked to cease consuming all products which contain aspartame. The study performs a double-blind experiment, where both of the participants and the testers do not know the test subject. They are randomly selected to take the aspartame or the placebo during the first and the second week periods. This is a good way to find out whether any different or same reaction of aspartame intake on those two different

The subjects refrained from caffeine, standardized diet, and standardized training for 48 hrs before the trials. For 24 hours before the trials, each subject was given a prepackaged standard diet. Exercise and food diaries were kept and checked for compliance.

Caffeine has many negative effects on humans, such as increased heart rate (Lane, J.D., 2002), depression (Goldstein, 2008), and addiction to this “drug.” You may be asking yourself, “What is caffeine?” Well, caffeine is actually a stimulant (Barone, Roberts, 2008) that is found in beverages such as tea, coffee, and soft drinks. In fact, caffeine is the highest grossing and most used stimulant in the United States (Barone, Roberts, 2008). It is estimated that 85% of adults living in the United States consume caffeine on a daily basis (Barone, Roberts, 2008). That means for every 100 adults, 85 of them have had a drink that contained caffeine on any given day. One reason caffeine is so widely available compared to other stimulants is