Chemical Evaluation Of Zinc Effects At 5 Ht1ar And Serotonin Neurotransmission
830 WordsOct 30, 20164 Pages
Our present study represents a further pharmacological evaluation of zinc effects at 5-HT1AR and serotonin neurotransmission in the context of zinc antidepressant-like activity. Since the role of postsynaptic 5-HT1AR in the pathophysiology of depression and in the action of antidepressants is widely discussed in the literature (see  for review), our present study evaluated the effect of zinc at postsynaptic 5-HT1AR in the PFC and Hp of rats after acute and chronic zinc treatments. Neither acute nor chronic zinc treatment induced significant changes in 5-HT1AR mRNA in the PFC or Hp (Fig. 1a, c). However, chronic zinc treatment increased protein levels of 5-HT1AR in the Hp by 78% (t=3.137, df=14, p=0.007) relative to control rats (Fig. 1d).
Discrepancies between 5-HT1AR mRNA and protein expression levels in different brain regions have been reported in animal stress models. Iyo et al., 2009 observed increased 5-HT1AR mRNA level and decreased protein level in the PFC . Our earlier animal studies showed no changes in 5-HT1AR mRNA in the PFC of female rats subjected to prenatal stress, in pregnant stressed female or in the Hp of male rats subjected to chronic mild stress . However, 5-HT1AR protein levels were reduced in these groups of rats . Shishkina et al., 2012  reported an increase in 5-HT1AR gene expression in the PFC of rats exposed to FST; this effect was reversed by chronic fluoxetine treatment . However, in the non-stressed rats, fluoxetine