Crusp Case Study

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The Propelling Factors of CRISPR’s Further Investigation The pioneer in the primary characterization of the CRISPR system was Francisco Mojica. In 1993, he was the first researcher to notice a pattern in a set of palindromic sequences. He was eventually able to correlate them with the genomes of certain bacteriophage. Following a more thorough investigation, he was able to confirm his hypothesis, and determine that the system was a function of the bacterial immune response. An unusual protein was located in the CRISPR locus by Alexander Bolotin in 2005, this protein was suspected to take part in nuclease activity. Following this new lead, scientists decided to focus their investigation on integrating the CRISPR system into human genome …show more content…

This grouping of bases sparked interest, and after further investigation they were given a name: Clustered Regularly Interspaced Short Palindromic Repeat, or CRISPR. Researchers were keen to understand and document CRISPR’s function in the biological world. While the sequences themselves were non-repetitive, their characteristics seemed to be. Further investigation showed that the genetic material of prokaryotes seemed to be laced with these unusual sequences. This spacing suggested that the newly discovered system was mobile. In addition to these intriguing discoveries, multiple genes were found to be tied to the repeats. With their interest peaked, researchers combed through the genetic material of several hundred types of bacteria searching for the sequences of foreign DNA. Their findings affirmed their theory, and that chapter of the CRISPR research project seemed to be coming to an end. Once the basic function of this system was mostly understood, researchers felt the need to move to towards applying this unique biological system to humans. The first step of this new ‘chapter’ was dependent on finding the true function of these swapped basepairs. The unusually arranged basepairs had an undiscovered, underlying cause. Base pair swaps in DNA along with integration of new genetic matter

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