preview

Effect Of Cell On Cell Mortality

Better Essays

Introduction Cell differentiation is the process that leads generalized cells to change patterns of gene expression and move towards the phenotype of specialized cells. Terminal cell differentiation occurs when a cell reaches its final specialized characteristics, and is usually where the cell permanently ceases division (Alberts et al. 2008). Some cell lines can be used to study differentiation. For instance, the PC-12 cell line differentiates in response to nerve growth factor by extending neuritic outgrowths from the cell body (Das et al. 2004). Nerve growth factor (NGF), a neurotrophin that regulates cell differentiation, plays an important part in various pathways and signals (Sofroniew et al. 2001). For instance, NGF plays an …show more content…

PC-12 cells, however, do not produce epinephrine (Greene and Tischler 1976). PC-12 cells differentiate reversibly in response to nerve growth factor (NGF) by forming neuritic outgrowths (Greene and Tischler 1976). The following study uses a derivative of the PC-12 cell line, called PC-12 Adh,, that shows increased adherence to Corning CellBIND® flasks which makes it easier to measure neuritic outgrowths. PC-12 Adh produces the same hormones as PC-12, but has not been vigorously tested for differentiation in response to NGF (ATCC). Yin et al. tested PC-12 and PC-12 Adh in response to NGF and found that both lines differentiated similarly within 48 hours (2015). This study will help to determine if the PC-12 Adh cell line differentiates similarly to PC-12. This would be helpful to researchers who want to use PC-12 Adh for its adhesive qualities but are unsure of how it will compare to results from PC-12 studies. These types of studies are also helpful for medicinal research. Past studies on the PC-12 cell line has offered insight into the mechanisms of the action of neurotoxicants and altering neuronal differentiation via chemicals (Das et al. 2004). Work on these cell lines may also be useful for research in neuronal injuries (Yin et al. 2015). The following study tests varying concentrations of NGF on PC-12 Adh cells to compare the two cell lines, and to quantify the effect that different concentrations of NGF have on

Get Access