Identification and Characterization of Somatic Mutations Associated with Progression of Acute Myeloid Leukemia Having FLT3ITD

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My thesis dissertation titled “Identification and characterization of somatic mutations associated with progression of Acute Myeloid Leukemia having FLT3ITD and screening of small-molecule inhibitors in treatment of AMLFLT3ITD” at Cancer Science Institute (CSI) of Singapore, National University of Singapore (NUS) was the ideal experience to acquire skills in molecular cloning, cell culture, protein biochemistry, bioinformatics and exome sequencing.

The molecular pathogenesis of Acute Myeloid Leukemia has not been completely deciphered yet but develops through a multistep acquisition of a wide range of somatic mutations. Therefore, investigation of cancer genome sequences and structures provide insights for understanding cancer biology,
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During this period, I was involved in two other projects on biology of Lamc2 in tumor progression of Anaplastic thyroid cancer and Pancreatic Cancer. The Thyroid Cancer study titled “Laminin-5 gamma-2 (LAMC2) is highly expressed in anaplastic thyroid carcinoma and is associated with tumor progression, migration and invasion by modulating signaling of EGFR” was published in the Journal for Clinical Endocrinology and Metabolism (JCEM) recently. I am studying the same mechanisms and pathway in Pancreatic cancer now. The pancreatic cancer project is also showing promising results and the paper is in preparation for publication.

After graduating in Genetic Engineering in August 2013, I was keen on exploring the field of developmental biology. I’m now an intern in the developmental epigenetics and diseases unit at Institute of Molecular and Cell Biology (IMCB), A*STAR, Singapore supervised by Dr Daniel M Messerschmidt on genomic imprinting during development of an organism. In this study, I’m studying the role of TRIM28 and its effects of knockdown in Embryonic Stem Cells. Dr Daniel Messerschmidt just started as a junior PI and I am the only member in his lab. Apart from experiments, I also manage laboratory supplies and utilities. This has given me an opportunity to work independently with minimal guidance.

Short-term projects and supplementary training during summer and winter of 2010,2011 and 2012 in Tumor induction (Induction of Hepatocellular carcinoma

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