Studies show that unwashed or lightly rinsed vegetables can harbor pathogenic bacteria and have been implicated in numerous foodborne infections. The objective of this experiment was to obtain a quantitative viable plate count of enteric bacteria present in a sample of broccoli sprout water. To enumerate the number of bacterial colonies present in the sample, we performed serial dilutions of broccoli water spread onto MacConkey Agar plates. MacConkey Agar is a selective and differential media. It is used to select enteric bacteria and differentiate lactose-fermenters from non-lactose fermenters. Utilizing this process illustrated the potential amount of foodborne illness causing bacteria present on foods, specifically fresh broccoli …show more content…
Sulforaphane is used for the treatment and prevention of several types of cancers as it inhibits the enzymes that create carcinogens and helps the body to metabolize and excrete them. Research shows that a handful of broccoli sprouts provide up to 50 times the amount of sulforaphane as one pound of fully mature broccoli, thus making them a popular alternative for meals to the fully mature plant. Studies are still being conducted as to the health benefits of broccoli sprouts, and there is conflicting information as whether they should be eaten raw or cooked for the maximum health benefit. Research shows that sulforaphane is sensitive to heat and thus several sources recommend that the sprouts be eaten raw to obtain the full nutritional value. (Broccoli Sprouts, n.d.). Additionally, some reports indicate that there is no risk associated with eating raw sprouts and that incorporating a small amount of sprouts into a daily balanced diet can protect against H. pylori, bacteria that is known to cause gastritis, ulcers, and stomach cancer. (Weil, A., MD., 2016). Broccoli sprouts are relatively easy to produce and can be grown in large quantities quickly with a minimal space requirement. Broccoli seeds can be grown in soil or placed in glass jars, soaked with water and placed in a warm, dark location to begin germination. It is recommended that the seeds be rinsed, drained, and re-watered every six to twelve
The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in
My unknown organism #6 is Morganella morganii, which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans, mammals, and reptiles as a normal flora. (3, 5) This bacterium Morganella morganii, was first discovered in the 1906 by a British bacteriologist named H. de R. Morgan. (2) Despite its wide distribution, it is an uncommon cause of community-acquired infection and is most often encountered inpostoperative and other nosocomial settings. (2, 3) Morganella morganii infections respond well to appropriate antibiotic therapy; however, its
An association between enzyme production, gene copy number, and gene evolution was explored by conducting analysis of the salivary amylase enzyme, AMY1A gene copy number, and the ancestral starch consumption in Homo Sapiens (Tracey 2017, p.22). It was hypothesized that the relative amount of starch consumption was very high for my personal ancestral diet, thus my AMY1 diploid gene copy number in my genome and salivary amylase concentration would be significantly higher than the population mean. With a population of 28 subjects (n=28), individual saliva samples were collected and compared to a calibration curve to determine the approximate amylase concentration by analyzing absorbance values. Individual samples of buccal cheek cells were
How should the unknown microscopic organisms be classified? The concept of the experiment was to conduct a systematic observation about the traits of unknown organisms, and classify them to the right group. Making detailed observations about the traits, made it possible to identify which cell the trait belonged to because different cells have different traits. Animal cells do not have a cell wall, and has one or more small vacuoles. Plant cells has a cell wall, rectangular (fixed shape), and has a chloroplasts. Cytoplasm, Endoplasmic Reticulum, Ribosomes, Mitochondria, and Golgi apparatus to name a few.
2. (5 pts) List and explain the names and affiliations of the various characters/stakeholders in this story – I’m looking for us to use the story to map out the complexities that are generally associated with solving public health puzzles – the stakeholders you list and explain here should apply to many of the cases we consider going forward.
The experimental procedures for Lab 2 were provided on Blackboard labelled as “Pre-Lab 2: Techniques & Measurement”.
The investigation is showing how enzymes work inside a mammal's stomach. Rennin is the enzyme found in young mammals and has more effect
Project 3 Formal Lab Report: The Identification and Analysis of an Unknown Department of Chemistry, Michigan State University Thursday October 19, 2017 Introduction An unidentified substance had been found in a teaching lab and its chemical properties have been declared unknown. The compound needed to be identified in order to be properly disposed of. The sample is known to be one of the twelve compounds given by the supervisors of the MSU Office of Environmental Health and Safety (EHS) as a baseline for the group of interns to build a compound profile. The samples that are under consideration are NaCl, KCl, Na2SO4, CaCl2, MgSO4, Na2CO3, K2SO4, KNO3, Ca(NO3)2, NH4CO3, NH4Cl, (NH4)2SO4, CaCO3, MgCO3, and
An unknown was given to our group from the professor. The unknown was in nutrient broth, the group received unknown number 3. And the task was to identify the unknown and try to make an educated guess, and identify the unknown #3.
Purpose: is to determine the unknown bacteria with a variety of biochemical tests. There are many reasons that contribute to why it is so important to test patients for both high and low risks diseases. The most important reason would be to know the identity of microorganism and how it can be treated .This study was performed in microbiology laboratory class by applying the microorganism to the tests that have been performed in the class prior to the identification of the unknown. First, the lab professor handed out a bacteria that was on the unknown streak plate labeled B5 that consisted of an unknown gram positive or gram negative bacteria.
Data were collected in September and November 2017 at the Elk Valley Preserve and Field Station in Banner Elk, NC. The preserve is located at 36°10'17.2"N 81°54'45.9"W, in Avery County in western North Carolina. The area is a mixed deciduous forest at an altitude of 1,127 m above sea level. Data were collected in early fall, during which temperatures average around 10-18 degrees Celsius. Summers in Banner Elk are typically mild, with temperatures averaging 21 degrees Celsius, and winters are cold and snowy, averaging near zero degrees Celsius. During the months of September and November there is an overall average precipitation of 12-25cm.
The petroleum lab and environmental reflect the major focus of the lab well. The abstract concisely communicates the purpose of the lab, the approach, the results, and the significances of the findings. The electrical lab doesn’t have an abstract but sometimes instructors do not require an abstract section. The environmental lab does a better job at writing the abstract because it’s shorter and more direct to the point, therefore easier to understand.
Hagen M, Ashraf M, Kim I, Weintraub N, Tang Y (2018) Effective regeneration of dystrophic muscle using autologous iPSC-derived progenitors with CRISPR-Cas9 mediated precise correction. Medical Hypotheses 110:97-100
Use a weigh boat and scoopula to measure out 0.75 g of agarose onto a digital scale. Make sure to tare the digital scale before weighing the powder. Pour the powder into a 125 mL Erlenmeyer flask.
I am writing this letter to you to inform and update our King’s Christian College Creek that is located in the middle of the school. As a class, we have conducted test to find the temperature, turbidity, pH, dissolved oxygen and found macroinvertebrates in the ecosystem. These tests has been conducted to figure out what we need to improve the ecosystem for a better habitat for the creatures living.