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Summary : Bluetongue Virus ( Btv )

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Summary

Bluetongue virus (BTV) is an arthropod borne virus causing ruminant haemorrhagic disease (MacLachlan, Drew, Darpel & Worwa, 2009). Typical presentation includes fever, mucosal erosion, ulcerations, and coronitis (MacLachlan, Drew, Darpel & Worwa, 2009). BTV has 24 serotypes, many topotypes, and can rearrange its double-stranded RNA genome to further increase variability. In 2006 an epidemic of BTV serotype 8 (BTV-8) caused a surprising amount of foetal infection. Consequently, this study aimed to investigate transplacental transmission of BTV-8 and compare incidence and consequences to BTV-1.

At 70-75 days gestation, nine pregnant ewes were inoculated with BTV-8 and conferred to isolation with one control. This was repeated for BTV-1. Bloods were analyzed using real-time reverse transcriptase PCR (RT-qPCR). BTV strains used were from the field; from a Netherlands’ ewe affected in the 2006 epidemic (BTV-8) and an affected Spanish ewe (BTV-1). 29 days after inoculation ewes were euthanized. Duplicate samples were taken from ewes and fetuses; for virus isolation as well as PCR (RT-qPCR and serotype specific) and ELISA testing.

All inoculated ewes at autopsy had BTV antibodies. The BTV-1 control ewe also developed antibodies. BTV-8 infection induced mild clinical signs where BTV-1 infection developed more severe signs. BTV-8 was demonstrated in 43% of fetuses where BTV-1 was demonstrated in 82%. Pathological changes were found in the central nervous system and in the

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