Introduction: Vitamin C, also known as ascorbic acid, is an important vitamin for a healthy diet. It is important in the growth and repair of tissue, skin, wounds, bones and teeth. It is also an antioxidant. Vitamin C is found in fruit juice and to discover how much vitamin C is in fruit juice, a titration can be performed. A titration is a lab method used to determine an unknown concentration of a solution by adding a reagent with a known concentration. The main goals of this experiment were to perform a redox titration and improve titration technique, to become familiar with the redox reactions with organic molecules, and to determine the amount of vitamin C in a sample of fruit juice.
Experimental: Part 1 of the experiment was the standardization of the Iodine solution. First, three ascorbic acid samples were weighed out to 0.10 grams each and then placed into a 250mL Erlenmeyer flask. Each flask containing the sample was then filled with 100mL of distilled water and the ascorbic acid dissolved. Next, 100 mL of the stock I2 solution was measured into a 400mL beaker and 150 mL of water was added and thoroughly stirred. A buret was obtained, washed thoroughly, and then rinsed three times with the I2 solution. Then the buret was filled with the I2 solution. 1 mL of starch indicator was added to the first ascorbic acid sample. An initial reading on the buret was recorded and then the titration began. The flask was gently swirled to mix the solution as the titration was
In this experiment redox-based titrimetric analysis was used to determine the amount of ascorbic acid (Vitamin C) contained in a serving of lemonade. This was done to confirm the 100 percent recommended daily allowance claimed on the package for a serving of Country Time Lemonade. The titration method used was adding a thyodene indicator to the analyte (lemonade solution) there is a visible colour change when the endpoint of the reaction between the titrant (KIO3) and the analyte occurs. The following reactions show how this titration method is viable. (Draw in reactions) KIO3 first reduced with iodide (I-) to generate iodine (I2 ) which then oxidized the ascorbic acid within the lemonade. Once all the ascorbic acid had reacted any iodine
The first thing that should be done when starting this lab is to write down observations on the materials that will be used in this experiment. After having written down the observations begin the lab by placing (without using your fingers) 2 small pieces of solid iodine into a small test tube that contains 1mL (20 drops) of water. Make sure to mix and crush with a stirring rod while commenting on how well the iodine is dissolving. Compare how well it dissolves in water by comparing how iodine dissolves in alcohol, mineral oil and a solution of potassium iodide. After doing that the solubility of zinc in water should be also checked by adding a few pieces of granular zinc into 1mL of water and then comparing how well it dissolves in water by
For every two moles of thiosulphate, one mole of iodine is needed so the amount of iodine mole needed is half the amount of thiosulphate moles that are needed. Starch is used as the indicator for the end point because the iodine and water is such a faint yellow color that the starch will combine with any of the iodine that is still present and turn it a blue color and the end point for the titration is than easier to notice.
HOW TO MEASURE THE ASCORBIC ACID CONTENT OF ORANGE JUICE A. A Fill a test tube with 10mL of indophenol. B With an eyedropper get some of the orange juice that is being tested. C One drop at a time, add the juice into the indophenol.
One of the drawbacks of using iodine is its low solubility in water, as well as its potential staining of teeth. One path to overcome these disadvantages is changing the drug delivery of iodine. Iodine complexed with polyvinyl pyrrolidone(PVP),to form povidone-iodine(PI), increase water solubility, decrease irritation and decrease the staining caused by pure iodine.(13)
Vitamin C also known as ascorbic acid is needed for growth and maintenance of healthy tissues, especially skin, blood vessels, bones, gums, teeth. Vitamin C aids in resistance against infection and healing of wounds. It also helps the body absorb iron from food. Vitamin C can be found in green vegetables, potatoes, tomatoes and citrus fruits such as oranges and lemon. A lack of vitamin C can cause scurvy, iron deficiency and poor wound healing. A healthy diet
Test tube rack Microliter pipette Iodine solution 6 pieces of Parafilm Starch solution Six buffer solutions (pH=4,5,6,7,8,9) Calibrated
My fourth report will be on the medical use of iodine. I will specifically focus on Iodine as a sterilization agent for surgeries or wounds in health care. Iodine is a chemical element used often in disinfectant found in the OR suite of a hospital.
The value of 82.5mg per 250mL serving will then be divided by 10 in, order to have the serving size to be equal to that of the experiment (25.0mL), resulting in the amount of Ascorbic Acid in the juice to be 8.25 mg, which is less than the observed and calculated value of 10.73mg. Thus there appeared to be random error within the
Added 300 ml of water to 400-500 ml beaker than added several drops of I2KI (iodine potassium iodide). until the water was visibly yellow. I2KI Than placed bag into the beaker for 30 minutes. Finally, the last procedure of this lab was the benedicts test this is commonly used to detect presence of reducing sugar. First they had to label 3 test tubes control bag and beaker, they added 2 pipettes of water for the control tube, 2 pipettes of the bag solution, and two pipettes of the beaker solution and added 1 drop of benedicts reagent to each tube.
[3]In this experiment, we are going to find out which fruit juice provides the most amount of vitamin C. We will use a blue substance called 2, 6-dichlorophenolindophenol (or DCPIP for short) as a reagent to test for vitamin C. It acts as an indicator where it changes from blue to pink with acids but loses its color in the presence of certain chemicals, one of which is ascorbic acid (vitamin C). DCPIP solution can be used to test for the presence of vitamin C in foods (but not for other vitamins which are entirely different kinds of chemical). It is an electron acceptor that is blue when oxidized and colorless when reduced. It is part of the Hill reagents family. DCPIP is commonly used as a substitute for NADP+. The dye changes color when it is reduced, due to its chemical structure. The nitrogen atom in the center of the molecule is the atom that accepts electrons, and it changes the double N-C bond to a single bond, which forces bonds between carbons in the entire left ring to change. This microscopic shift in the DCPIP structure causes the macroscopic change in color, from dark blue to colorless. If vitamin C, which is a good reducing agent, is present, the blue dye, which turns pink in acid conditions, is reduced to a colorless compound by ascorbic
Vitamin C is crucial for survival for several reasons. For a human body to reach the acme of its physical potential, it must have a daily intake of vitamin C. Deficiency can lead to a fatal symptom known as scurvy. Likewise, iron absorption will be more difficult resulting in iron-deficiency anemia (Smith, Primio & Humphrey-Murto, 2011). However, this nutrient is essential for bodily functions as well. Vitamin C is required for hormone production which is seen by the fact that the highest concentration of it in the human body is in the pituitary and adrenal glands (Emsley, 2004). Moreover, this vitamin aids in protection against free radicals and stress. Vitamin C is also imperative because it assists in the growth of various body parts, including teeth, cartilage, and dentine. This acid is integral for bone development and to heal fractured or broken bones. As said before, “Vitamin C is important in the absorption and metabolism of many nutrients that affect production of red blood cells”, including iron absorption (Smith, Primio, & Humphrey-Murto, 2011). DIRECT QUOTE WITHOUT AUTHOR. Similar to its importance to humans, vitamin C is vital for plant life as well. Plants require the alcohol for growth processes.
Vitamin C, or ascorbic acid, is a well-known supplement that is essential to the human body. Vitamin C helps grow and repair body tissue, make collagen, heal wounds, and strengthen bones and teeth. Unfortunately, the body does not produce this vitamin itself, therefore it must be obtained from another source. Vitamin C is present in significant amounts in both fruits and vegetables. These foods are commonly pasteurized – a process that applies heat to destroy pathogens that cause spoilage in food. Pasteurization is great for preserving foods, but its effects on the food’s contents are important to consider. This process could affect the levels of ascorbic acid in the foods being consumed for their vitamin C content. Specifically, orange juice, one of the most popular sources of vitamin C, is going to be used to examine the effects of pasteurization on ascorbic acid levels in this experiment.
A single service of orange juice has more than 200% of the vitamin C requirement of the body. Vitamin C, also known as ascorbic acid, functions as a primary antioxidant in the body, and it destroys or neutralizes free radicals before they can do damage to the body’s systems. Our bodies need vitamin C to stimulate other aspects of our immune system as well. Furthermore, ascorbic acid is one of the fundamental components of collagen, which is necessary to repair cells and for the growth of new tissues in the