Abstract
Faster and uniform germination of seed as well as establishment of vigourous crop is of paramount importance in direct-seeded rice crop, which in turn depends on seed germination and vigour per se. Rice being an albuminous seed, it is hypothesized that galactomannan hydrolysing enzymes (viz., β-mannanase, β-mannosidase and α-galactosidase) play important role in loosening of the embryo-surrounding tissue viz., endosperm, which in turn governs the speed of germination. The study was an assessment of this hypothesis, on ten rice genotypes comprising of five early germinating (which have taken less than 30 hours for radicle emergence) and five late germinating (which have taken more than 44 hours for radicle emergence) genotypes. The effect of gibberellins (GA) and Abscisic acid (ABA) on enzyme activity was also subsequently evaluated. The activities of β-mannanase, β-mannosidase and α-galactosidase were present in dry and germinating rice seeds and increased during germination. GA could promote the activities of all the three enzymes that reduced the time taken by the germinating seed to reach the stage of lemma rupture and radicle emergence. On the other hand, ABA had an antagonistic effect on the activities of these enzymes and enhanced the time taken by the germinating seed to reach the stage of radicle emergence. However, no changes were observed with respect to time taken for lemma rupture, which corroborate that lemma rupture could be due to expansion of
The hypothesis behind this experiment is that the Gibberellic acid has a positive growth effect on the plant and causes it grow larger in height.
When planting a seed, many factors allow the seed to grow and become a plant. To sprout from the ground, and to survive, seeds need water, air, and a certain temperature. Seeds can get water, oxygen, and sun by being placed at the proper planting depth. Planting depth is the depth at which a seed is placed in the soil. If a seed is exposed to these important needs, it goes through a process called germination. According to the Wise Geek article, “What is Germination?”, “Germination is a process in which a seed or spore awakens from dormancy and starts to sprout.” (“What is Germination?”).
The first lab was conducted to analyze how germination affects the rate of cellular respiration in lima beans compared to dormant seeds. In order to
The results obtained are non-conclusive. More research is necessary in order to fully understand the effects of Nitrogen in the development of Fast Plants seeds and the soil. It is recommended that original is repeated. However, only one fertilizer should be added per quad, rather than mixing the fertilizer used for the control with the fertilizer being studied. For future studies it is also recommended to maintain a record of the pH of the soils before, during, and after the experiment in order to understand the impact of fertilizers on the
Round seeds (R) are dominant to wrinkled seeds (r), and yellow seeds (Y) are dominant
Cellular respiration is going on in every cell in plants. It is interesting to know there is several of factors that affects cell respiration in plants for example ph. levels, temperature, oxygen, sugars. Germinating seeds carry out cellular respiration processes in much the same way as plant and animal cells do (Jeanty). Plant seeds respiration requires enough to maintain food or nutrients. The experiment that was done is testing the rates cellular respiration of germinating lentils that were soaked in different liquids which are water, juice, cola, Gatorade and milk. The hypothesis one is the highest rate of respiration is going to be the germinating lentils in water. The second highest rate of respiration is juice. The third is Gatorade. The fourth highest is milk. The lowest rate of respiration is going to be with the germinating lentils in cola.
The second step of the experiment was to soak the seeds in water overnight. This action was made to prepare the seeds for germination and making them more softer and less rigid. The seeds were placed in a bowl and were covered by tin foil. It was set up on the refrigerator to minimize any outside interference that may come to it. After a full night of absorbing the water, the seeds were ready to start the next stage.
To calculate the amylase activity in the germinating barley (2g), we needed to know the amount of 0.5% starch used in the reaction tube (5mg) and the time taken to reach the achromic point, to get the average amount of starch hydrolysed / min to reach achromic point for 1mL of Diluted Amylase Extract(DAE), thus, we could get average amount of starch hydrolysed / min to reach achromic point for 25mL of DAE, which was same as the average amount of starch hydrolysed / min to reach achromic point for 5mL of amylase, therefore, we got the average amount of starch hydrolysed / min to reach achromic point for the total volume of the filtrated, non-diluted amylase extract, with knowing the weight of barley grains/germinant used to make the extract, we could calculate the amylase activity as mg starch hydrolysed/min/g barley tissue.
In conclusion to the experiment, it was discovered that all of the substances being tested showed respiration except for the non-germinating peas. The trends in the most of the graphs showed the level of oxygen decreasing while the amount of carbon dioxide increasing. In comparison to the other groups, the overall trend was similar although some groups did not follow the exact protocol. The cold germinating peas inhibited oxygen consumption, however they were still respiring since they had to regain energy from their cold environment. According to The Journal of Research in Science and Teaching, it is important to understand that this photosynthetic process relates to cellular respiration because photosynthesis creates the glucose that is
Aerobic respiration is the way toward delivering cellular energy involving oxygen. Cells separate the food in the mitochondria in a long, multistep prepare that produces 32 ATP. The initial phase in is glycolysis, the second is the citric acid cycle and the third is the electron transport system. Germination is one of such procedures that make use of aerobic and in light of the fact that specific variables can influence aerobic respiration, it would likewise influence germination. The chemical reaction for aerobic respiration is:
Purpose: To see the effect of an acid introduced during seed germination, on the length of the plant roots. Also shows how salt can affect the seed germination. Acid can be introduced to seeds during germination if there is acid rain. Knowing the results of acid rain on seed germination will help us understand how to grow pants better, and how to have more successful germination. Salt can be introduced into a seed during germination because salt is put on roads and the salt builds up and can contaminate the soil. Knowing the effects of salt on seed germination will allow us to know for sure if the salt is affecting the plants growth or not.
In the year of 2000, the first genetically modified rice was successively introduced into the world by two scientists, Ingo Potrykus of the Swiss Federal Institute of Technology and Peter Beyer of the University of Freiburg. The original name of the Golden Rice was called SGR1. The Golden Rice is genetically modified so that vitamin A is in every grain of Golden Rice. Although the rice plant can naturally make the beta-carotene, a precursor of vitamin A, it only occurs in the leaf of the rice plant during photosynthesis. However, for the scientists to make the Golden Rice they had to insert the genes of a daffodil (phytoene synthase) and a gene from a soil bacterium called Erwinia uredovora (carotenoid desaturase) into the rice nuclear genome and under the endosperm promoter. By putting the genes in the promoter of the endosperm, the Vitamin A was able to be
Genetic engineering (GE) has proven to be the consistent approach for biofortification due to the limitless diversity available within the specific gene pools of the staple foods and can, therefore, be implemented directly to cultivars without the complex breeding programs (Farre et al., 2011). This allows for the GE of the staple crops that can produce higher nutrient levels than those in conventional breeding. However, most of these engineered varieties are still at the laboratory testing phase or in early field trials (Arjó et al., 2012). One of the most known variety is the Golden Rice, which was engineered with two genes with the ability to synthesize β-carotene (pro-vitamin A) in the seeds (Ye et al., 2000). In plants, the biosynthesis of pro-vitamin A is turned on
The purpose of the project was to determine whether Eucalyptus globulus had an effect on the germination of radish seeds. It was hypothesized that Eucalyptus globulus would have a negative effect on the germination of the seeds and their growth.
Modern genetic engineering (GE) and plant hybridization can trace its roots to a 19th century Austrian monk named Gregor Mendel, whose pioneering studies on garden pea plants revolutionized the comprehension of how heredity and the transmission of genetic traits function (Schwarzbach, Smykal, Dostal, Jarkovska, & Valova, 2014). However, it was not until Watson and Crick (1953) that the double helix structure of DNA and the composition of chromosomes were identified. With this knowledge, scientists then sought novel ways to more efficiently manipulate an organisms’ genetic sequence, eventually leading to a California-based company named Calgene to introduce the first genetically modified (GM) food, the Flavr-Savr tomato, to the consumer market in 1994 (Redenbaugh, Hiatt, Martineau, & Emlay 1994). Genetic engineering is largely carried out by recombinant DNA technology and allows scientists “to add one or a small number of genes from essentially any organism to simple bacterial cells” (Knight, 2015). Once genes are inserted into the bacterial cells, they can then be introduced into another organism. Foods have been genetically modified, since the production of the Flavr-Savr tomato, to serve specific functions. The functions most closely associated with GM foods have been “improved seed yield, shorter stems that better resist wind damage, herbicide resistance, frost resistance, salt tolerance” (Bennett et al., 2004).