Materials and methods Seeds Eighteen seed lots from six varieties that produced in three consecutive years (2012 to 2014) gathered from different climates of Iran (Table 1). All of those stored in very different condition from unprotected place to the cooled storehouse. Preliminary Seed Vigour Testing The standard germination protocol for the between paper method (ISTA 2008) was used to evaluate four replicates of 100 seeds from each seed lot at four temperatures: 20°C, 17°C, 15°C and 13°C in the dark. Mean radicle emergence time (MRET), mean germination time (MGT) and standard germination (SG) were calculated and compared with field emergence (FE) and mean emergence time (MET) measured in the field. In order to evaluate MRET and FE, the …show more content…
The containers of towels were put a transparent box and placed in a tied plastic bag and held in an incubator at all temperatures ± 0.5°C. The placement of the boxes was rotated in the incubator after each measuring. Counts of germination for six chosen six seed lots were done every 6 h for 4 days at 20°C and every 6h for 4 days at 20°C. Counts were done for just germination (JG or RE), that is, when the radicle first appeared through the pericarp and physiological germination (G) when a 2mm radicle had been seen. Two measures of the relative rates of germination were calculated: 1. Mean just germination time (MJGT) = Σ (nt) / Σ n, where n = number of seed newly germinated (just germinated criterion) at time t; t = days from seeding to germination. 2. Mean germination time (MGT) was calculated using the same formula, but using the physiological assessment of germination (2mm radicle). The MJGT and MGT were assessed for each replicate and used to create a mean for each seed lot. Only seeds that finally produced normal seedlings (ISTA, 2005) were included. All that tests were performed at 20°C to 17°C, 15°C and 13°C temperatures in order and in the same way sequence, fully implemented. Imbibition After identifying relationships between MJGT, MGT and Germination at different times and temperatures, 15°C were chosen for imbibition testing because it was the best match with field tests' results. Water uptake and
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In the dishes, I dropped the appropriate treatment into the center, where the marks were made. Next, I closed the petri dishes, taped them up, and let them sit at room temperature for a week. Then I opened them up to take two measurements. The first measurement was the number of seeds germinated. The second measurement was to measure the seedling lengths.
The experiment was begun by obtaining four 8 oz. Styrofoam cups and punching a hole through the bottom of them. This hole was for water entry or excess water drainage. Moistened soil was packed to the 1/2 full line in the cup along with 3 fertilizer pellets The cups were labeled the following: Rosette-H20, Rosette-GA, Wild-Type-H2O, and Wild-type- GA.(Handout 1) A small wooden applicator stick was obtained a moistened at the tip with water from the petri dish labeled ‘water.’ This was to be able to attract the seed to the applicator in order to place the seed from its original container into
For my seed experiment I had decided to see what the effect of sprinkling salt on a radish seed would be. So for my control group I had set six (6) cherry radish seeds in between a damp paper towel and then closed it within a Ziploc bag. For my experimental I had set it up the exact same way as the control group but I would sprinkle salt on top of seeds before I zipped up the baggie. I sprinkled the salt on the experimental seeds and dampened the paper towel once every day. Each bag was stored in the light and at room temperature (~70° F.)
The purpose of this experiment is to observe the effects of Sodium Chloride (NaCl) on the germination rate of Wisconsin Fast Plants.
When planting a seed, many factors allow the seed to grow and become a plant. To sprout from the ground, and to survive, seeds need water, air, and a certain temperature. Seeds can get water, oxygen, and sun by being placed at the proper planting depth. Planting depth is the depth at which a seed is placed in the soil. If a seed is exposed to these important needs, it goes through a process called germination. According to the Wise Geek article, “What is Germination?”, “Germination is a process in which a seed or spore awakens from dormancy and starts to sprout.” (“What is Germination?”).
The results observed do not correspond with the outcome predicted by the hypothesis. Despite the nature of the subjects of the experiments, no substantial growth was observed. Only one seed of the 36 planted germinated, and it could only survive for a period of a week. The one seed that germinated reach a height of 1.2 cm. Table 1 presents the average growth observed in each quad. Each quad had a total of 12 seeds. No seeds were removed during the course of the experiment.
Firstly, for the setup of the experiment, two styrofoam cups were filled with two inches worth of standard, fertilized garden soil, next four seeds from from the garden seed, and the bird seed were placed an inch deep in separate cups. The seeds were blindly labeled, with one being labeled group A and one being labeled group B. This was so as to efficiently conduct a double blind experiment. The seeds were watered with approximately a teaspoon of water per day, and kept in a sunny windowsill. They were left in the windowsill for two weeks, and watered daily.
Therefore, I was correct in my hypothesis that dark will have an effect on the germination of radish seeds. Also, I was very close in predicting that the seeds grown in the light will germinate twice as much as the seeds grown in the dark; the control seeds grew a little less than double the size of the experimental seeds. Ultimately, my experiment proved that light is a very important factor in the growth of any plant. However, I’ve learned that seeds grown in the dark will germinate, though slowly, as
This experiment was performed to give a better idea of interspecific competition and intraspecific competition between radish seeds and wheat seeds at high and low densities. By planting two species only pots and two combined species pots our results showed that the radish seeds performed better at both interspecific and intraspecific competitions and concluded that the lower the pot density the more resources and growth. 32 radish seeds were evenly planted in pot A1 followed by 32 wheat seeds planted in pot A2 and 16 of each radish and wheat seeds combined (32 total) planted in pot AB1. We repeated this procedure again but this time planting 96 radish seeds evenly in pot A2, 96 wheat seeds in pot B2 and 48 of each radish and wheat seeds combined (96
After this, 25 germinating peas were placed in Respirometer vial 1, 25 dry peas were placed in Respirometer vial 2, and the equivalent volume of beads in Respirometer vial 3. These step were repeated for the corn and beads for Respirometers 4-6. A rubber stopper with a calibrated pipet was put into each respirometer vial tightly so the respirometers did not
The second step of the experiment was to soak the seeds in water overnight. This action was made to prepare the seeds for germination and making them more softer and less rigid. The seeds were placed in a bowl and were covered by tin foil. It was set up on the refrigerator to minimize any outside interference that may come to it. After a full night of absorbing the water, the seeds were ready to start the next stage.
Purpose: To see the effect of an acid introduced during seed germination, on the length of the plant roots. Also shows how salt can affect the seed germination. Acid can be introduced to seeds during germination if there is acid rain. Knowing the results of acid rain on seed germination will help us understand how to grow pants better, and how to have more successful germination. Salt can be introduced into a seed during germination because salt is put on roads and the salt builds up and can contaminate the soil. Knowing the effects of salt on seed germination will allow us to know for sure if the salt is affecting the plants growth or not.
The purpose of the project was to determine whether Eucalyptus globulus had an effect on the germination of radish seeds. It was hypothesized that Eucalyptus globulus would have a negative effect on the germination of the seeds and their growth.