Use of Maggots for Wound Care

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Orthopaedic Surgery (2010), Volume 2, No. 3, 201–206


Clinical research on the bio-debridement effect of maggot therapy for treatment of chronically infected lesions os4_87 201..206

Shou-yu Wang MD1, Jiang-ning Wang MD2, De-cheng Lv MD1, Yun-peng Diao PhD3, Zhen Zhang MD1

Department of Orthopedic Surgery, The First Affiliated Hospital, 3Department of Pharmacy, Dalian Medical University, and 2Institute of Reconstructive Surgery, Dalian University, Dalian, China

Objective: To evaluate the bio-debridement effect of maggot therapy for treating chronically infected lesions. Methods: A retrospective study was conducted of 25 patients with diabetic foot ulcers and 18 patients with pressure ulcers after spinal
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The patients who were agreeable to maggot therapy were required to sign an informed consent form.

Preparation of maggots
Firstly, eggs were collected from the eyes of Scomberomorus niphonius and disinfected in 1% sodium sulfite solution for 3 min, and subsequently in 3% Lysol brand disinfectant for 5 min. The disinfected eggs were then transferred to sterile vials to clone. Secondly, third stage larvae of Lucilia sericata were selected to be placed in 3.5% formalin for 5 min, 2% hydrogen peroxide solution for 3 min, and then 5% dilute hydrochloric acid solution for 5 min. After the two-step disinfection, the larvae remined vigorous. A hundred randomly selected larvae were proven to be aseptic by bacterial culture test.

After two-step disinfection, disinfected larvae were applied to the lesion.
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