Yeast Fermentation Lab Report
SBI4U
Chaweewan. Sirakawin
Present to Ms.Allinotte November 21. 2014
Introduction: Fermentation is a metabolic pathway that produce ATP molecules under anaerobic conditions (only undergoes glycolysis), NAD+ is used directly in glycolysis to form ATP molecules, which is not as efficient as cellular respiration because only 2ATP molecules are formed during the glycolysis. One type of fermentation is alcohol fermentation, it produces pyruvate molecules made by glycolysis and the yeast will break it down to give off carbon dioxide, the reactant is glucose and the byproducts are ethanol and carbon dioxide. In this lab, the purpose is to measure whether the changes of
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2) The three bottles were labeled
1. 5 mL sugar
2. 10 mL sugar
3. 15 mL sugar
3) 150 mL of room temperature water was added to each 3 bottles
4) One package of active dry yeast was added to bottle labeled ‘5mL’ and solution was swirled.
5) 5 mL of sugar was added to the solution and a balloon was placed over the opening of the bottle to minimize the loss of any gas from the system.
6) The tape was used to measure gas accumulation in the balloon after 1minute. Measurement and qualitative observations were recorded.
7) The gas accumulation in the balloon was measured and recorded at one minute intervals for a total of 10 minutes (qualitative observations were included)
8) One package of active dry yeast was added to the bottle labeled ‘10 mL sugar’ and solution was swirled by rod gently.
9) 10 mL of sugar was added to the solution and the balloon was quickly placed over the opening of the bottle to minimize the loss of any gas from the system.
10) The tape was used to measure gas accumulation in the balloon after 1minute. Measurement and qualitative observations were recorded.
11) The gas accumulation in the balloon was measured and recorded at one minute intervals for a total of 10 minutes (qualitative observations were included)
12) The 8-11 steps for each of the remaining bottle was repeated, the sugar concentration was adjusted accordingly.
Observation:
The bottle with higher concentration of sugar tends to produce more carbon dioxide. After 10 minutes, The
of sugar to 25ml of water and dissolve it. When we have the water at
___The helium in the tank must be more dense than the helium in the balloons. ______________________________________________________________________
C) Again, rinse the saucepan and then add 250 grams of sugar to your 250ml graduated cylinder and then add water up to the 250ml mark. You will place a small piece of plastic wrap over the top of the graduated cylinder (or parafilm if you have that), and mix the sugar with the water. Then pour the contents into a small saucepan over the stove. You will fill the graduated cylinder up to the 250ml mark again and then pour the tap water into the saucepan as well. You will now heat the mixture on the stove and stir until the sugar has dissolved. Once this has happened, you will remove the solution from the saucepan, pour the solution into a container and label that container 50% sugar solution.
Abstract: This lab’s purpose was to see how different levels of yeast, distilled water, and sugar interact to affect the level of carbon dioxide evolved in fermentation. In this experiment we had two sections. The first section tested four test tubes with varying levels of yeast, glucose and distilled water for evolved carbon dioxide levels. The tubes were timed for 20 minutes. The amounts of solution in the test tubes are noted in the methods section of this lab report. The second section of the lab used three test tubes and flowed the same procedure except added spices. The levels of ingredients are also in the methods section. The main goal of this experiment was to see the effects of yeast concentration.
No significant differences between the mean hang times could be found. In the second experiment, the researcher found that helium and nitrogen, gases lighter than air, had longer hang times than air, while carbon dioxide and argon, gases heavier than air, had shorter hang times (see Table 2 and Graph 2). There were significant differences between the mean hang times. Both results were subject to statistical analysis. An ANOVA and a follow-up Tukey HSD test were used on both results in the stat software JMP. In the first experiment, the ANOVA and Tukey HSD test resulted in p-values all over 0.05, which means there were no statistical differences between the mean hang times. In the second experiment, the ANOVA and Tukey HSD test resulted in p-values all under, 0.05, which means there were statistical differences between the mean hang
3. Bring the charged balloon close to the paper scraps from Part 2. How does the rubbed balloon
8.When the reaction is completed, record the volume of gas in the graduated cylinder. Record observations about which reactant was the limiting reactant.
2. Fill a flask with pure water (0% sugar), another with 0.2M water, another flask with 0.4 M water, and another flask with 0.8M water.
2. Note that the balloon in the chamber is filled with 0.300 moles of an
In Boyle’s experiment to see how pressure and volume were connected, Boyle used a J-shaped piece of glass tubing that was sealed on one end, gas was trapped in the sealed end of the tube and fluctuating amounts of mercury were added to the J-shaped tube to vary the pressure of the system. Boyle fluctuated the pressure and measured the
in the gas mixture was measured in a controlled way using a RH sensor and is shown in figure~\ref{fig:6_Water_vapor_quantity}. Throughout
When we put gas inside the balloon the gas was evenly spread out making sure that the pressure was constant. This follows the Kinetic Theory of Matter because it explains that the particles of a substance are in a constant motion.
3 Add one textbook to the center of the small wood platform and record the new volume of the air that is occupying the cylinder. Record the pressure always adding 2.4 tbs to how many tbs used to account for the atmosphere. If necessary, gently hold the book in place.
The following report explains the results of a model hot air balloon experiment that replicates a previous incident in which the temperature in the envelope was heated to a point until it was burned. After assessing the researched and calculated data, the assertion of the “air in the balloon becoming too hot” to lift the mass will be validated.