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1. Describe the requirements for growing mammalian cells in vitro.
2. Explain the difference in read-out between the Crystal Violet Dye Elution Assay and the
Acid Phosphatase Assay. Which assay is more reliable? Provide justification for your
answer.
Step by step
Solved in 3 steps
- 2. Explain the difference in read-out between the Crystal Violet Dye Elution Assay and the Acid Phosphatase Assay. Which assay is more reliable? Provide justification for your answer.1. Labels are used in biosensors to amplify a biorecognition event. Which is frequently used in lateral flow assays? C-13 or P-32 nanoparticles fluorophores labeled enzymes 2. Why is it a good idea to use filters? Because of the mains interference All of these Because of the parasitic Because the signal is mixed with other unwanted signals3. Describe the Fehling's test. What does the Fehling's test detect?
- 1. Propose a way that a chi square analysis could be used in other experiments, such as genetics ordrug trials.Discuss and explain the results of this graph. Graph shows results of WST-1 assay. T0 ( time zero) shows assay performed in order to obtain an absorbance at the time of test agent added in order to determine 1. how much cells have grown over the incubation period 2. Growth inhibition by test agent 3. If test agent caused cytotoxicity1. If a negative control produces a band, what does this indicate? 2. In an experiment, a student’s sample amplified for D1S80 produced 3 bands. It was the only DNA sample run on the gel. The student knows that there was no problem with the Thermocycler or primers because the other students in the class had the expected results of only one or two bands. What is the most likely explanation for these results?
- 1.a)What is the equivalence point and how does it relate to the recommended proportion of serum to blood in the heme agglutination assay? b. what would the predicted outcome be if you used too little serum in this assay? Why? c. what would the predicted outcome be if you used too much serum in assay? Why?For tha analysis of a specific drug present in different pharmaceutical preparations, explain when is more appropriate to use absorbtion based assay and when is more appropriate to use a fluorescence based assay.Following on from the last question, what assays could you do to find out if your mitochondria are pure? Select all that apply A) Assay succinate dehydrogenase to confirm mitochondria are present B) Assay alkaline phosphatase to check for contamination with Lysosomes C) Measure protein using the Bradford assay D) Assay acid phosphatase to check for contamination with Lysosomes E) Assay lactate dehydrogenase to confirm mitochondria are present
- What are the different categories of cell viability assays? Describe one of them briefly. Define the role (aim of use) of Trypan blue dye in cell culture studies.Please offer a clear explanation of the ChIP assay. A. What characteristics of these cells are being assessed? Why would this be valuable to a research team? B. Define assay and explain how samples are managed step by step with a clear explanation of each step. C. What type of data is being generated by this experiment?With detail, compare and contrast the following 5 real-time assays; Taqman, SYBR Green, Molecular Beacon, FRET, and Scorpion. Please write complete sentences in paragraph form. Identify how the assays are similar but more importantly, what are the distinguishing features of each of the technologies.
