Read the article in the link and do a data analysis. What are the results and findings of the study?https://mdpi-res.com/d_attachment/ijms/ijms-21-05129/article_deploy/ijms-21-05129.pdf?version=1595262192 Notas 1:48 p. m. Vie 29 sep.2. Results2.1. Flow Cytometry with Canine CD MarkersFlow cytometry analyses confirmed that the population of BMMSC expresses a low number ofsurface markers typical for hematopoietic cells (CD45), while expression of surface markers typicalfor MSCs (CD29 and CD90) is high. The results presented in Figure 1 show that cells at passage 3expressed primarily CD29 (88.5 ± 2.2%) and CD90 (77.3 ± 4.6%) and the proportion of CD45+ cells waslow (3.0 ± 1.7%).Int. J. Mol. Sci. 2020, 21, 5129Amdpi-res.comCD90 APC-AB1010CD29 PE-AC MSC canine CD29/45/90-BM02/CD29/45/91 D MSC canine CD29/45/90-BM02/CD4/8/21CD90+IgGMSC canine CD29/45/90-BM02/CD29/45/9CD29+IgGALACD45 FITC-AIgGCD45+3 of 21Figure 1. Immunophenotyping of bone marrow tissue-derived MSC (BMMSC) at passage 3. (A) Noteconfluent population of BMMSC revealing spindle-shaped morphology. (B-D) Flow cytometry analysesof BMMSC expressing high percentage of mesenchymal markers CD29+ (88.5 ± 2.2%) and CD 90+(77.3 ± 4.6%) and low percentage of hematopoietic markers CD 45+ (3.0 ± 1.7%). Scale bar A = 50 μm.2.2. Multilineage PotentialThree-lineage potential was confirmed using the commercial StemPro® Differentiation Kit andcorresponding protocol for induction towards chondrogenic, osteogenic and adipogenic lineages.After 14 days of incubation in cell-type corresponding differentiation medium, we confirmed thatcanine BMMSC show a high degree of biomineralizing osteogenesis with visual staining of calciumdeposits expressing Alizarin Red positivity (Figure 2B). Furthermore, chondrocytes migrating fromspherical chondrocyte-like aggregates revealed intense Alcian Blue staining of glycoproteoglycans(Figure 2D). In contrast to the high osteogenic and chondrogenic differentiation of canine BMMSC,Commation and Oil Rai stainime found a low dewith limite38 %

The study delves into the fascinating world of canine bone marrow-derived mesenchymal stem cells…

2. Results 2.1. Flow Cytometry with Canine CD Markers Flow cytometry analyses confirmed that the population of BMMSC expresses a low number of surface markers typical for hematopoietic cells (CD45), while expression of surface markers typical for MSCs (CD29 and CD90) is high. The results presented in Figure 1 show that cells at passage 3 expressed primarily CD29 (88.5 ± 2.2%) and CD90 (77.3 ± 4.6%) and the proportion of CD45+ cells was low (3.0 + 1.7%). Int. J. Mol. Sci. 2020, 21, 5129 B CD90 APC-A Count obi MSC canine CD29/45/90-BM02/CD29/45/9 CD29+ IgG D CD29 PE-A C MSC canine CD29/45/90-BM02/CD29/45/91 D MSC canine CD29/45/90-BM02/CD4/8/21 -CD90+ - IgG MODE CD45 FITC-A IgG CD45+ 3 of 21

2. Results 2.1. Flow Cytometry with Canine CD Markers Flow cytometry analyses confirmed that the population of BMMSC expresses a low number of surface markers typical for hematopoietic cells (CD45), while expression of surface markers typical for MSCs (CD29 and CD90) is high. The results presented in Figure 1 show that cells at passage 3 expressed primarily CD29 (88.5 ± 2.2%) and CD90 (77.3 ± 4.6%) and the proportion of CD45+ cells was low (3.0 + 1.7%). Int. J. Mol. Sci. 2020, 21, 5129 B CD90 APC-A Count obi MSC canine CD29/45/90-BM02/CD29/45/9 CD29+ IgG D CD29 PE-A C MSC canine CD29/45/90-BM02/CD29/45/91 D MSC canine CD29/45/90-BM02/CD4/8/21 -CD90+ - IgG MODE CD45 FITC-A IgG CD45+ 3 of 21

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter4: Basic Immunology And Immunohematology

Section4.4: Tests For Human Chorionic Gonadotropin

Problem 7RQ

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Yasuo Yamagata, a 63-year-old, experienced severe back pain for several weeks before visiting his family physician. He also complained of fatigue and looked pale. Blood analysis revealed a red blood cell count of 3.2 × 106/μl (normal 4.2–5.0 × 106/μl), a white blood cell count of 2800/μl (normal 5000/μl), a sedimentation rate of 30 mm/h (normal <20 mm/h) and a serum IgG of 4500 mg/dl (normal 600–1500 mg/dl). IgA and IgM levels were well below normal. Skeletal survey showed lytic lesions in vertebrae, ribs and skull. A bone marrow sample revealed 75% infiltration with plasma cells. Elevated protein in urine was confirmed to be Bence-Jones protein (immunoglobulin light chains). The patient was diagnosed with IgG λ multiple myeloma and began an immediate chemotherapy regime. Which of the following would be consistent with this type of malignant tumor of plasma cells? a. Serum IgG is polyclonal. b. Anemia and neutropenia are present as the result of plasma-cell infiltration in the…
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