3. DNA polymerase made a mistake and added a C on the DNA template strand. In the space on the mRNA sequence below, write the added base. (Remember that the DNA template and mRNA are complementary. Mark the codons again and write the amino acid sequence beneath them. What do you observe? (5') CGUUACAAUGUAU_CGCGCGGUACUCGGCAAAGUGCCCUGAAUAGAGUUGGUA 3' On this mRNA codon table, the first nucleotide in codon is to the left, the second is above, and third is to the right. the omingood tRNA U U Phe Phe Leu C Ser Ser Ser A G Tyr Cys U Tyr C Cys STOP STOP A

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Chapter12: Dna: The Carrier Of Genetic Information
Section: Chapter Questions
Problem 8TYU: The statement DNA replicates by a semiconservative mechanism means that (a) only one DNA strand is...
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3. DNA polymerase made a mistake and added a C on the DNA template strand. In the space on the mRNA sequence
below, write the added base. (Remember that the DNA template and mRNA are complementary. Mark the codons again
and write the amino acid sequence beneath them. What do you observe?
(5') CGUUACAAUGUAU CGCGCGGUACUCGGCAAAGUGCCCUGAAUAGAGUUGGUA 3'
On this mRNA codon table,
the first nucleotide in codon is to the left,
the second is above, and third is to the right.
4. A mutation in the gene encoding the aminoacyl tRNA
synthetase for valine (VAL) causes the tRNA binding site to
have the wrong shape. In its new (mutant) shape, the enzyme
can bind tRNAs for VAL and leucine (LEU). (not at the same
time but enzymes work fast and there are lots of them)
a. In cells with this mutant tRNA synthetase, will the
protein product of translation match the original one
you deduced in question 2? circle one: (YES / NO. )
b. If not, how does it differ?
c. How many different version of the short polypeptide
will exist in this mutant cell?
U
U Phe
C
Ser
Alle
lle
lle
Met
G Val
Val
Val
Val
A
Tyr
Tyr Cys
G
Cys
Phe Ser
Leu
Ser
Leu
Ser
STOP Trp
C Leu
Pro
His
Leu
Pro
His
Leu
Pro
Gin
Leu
Pro Gin
Thr Asn
Thr
Asn
Thr Lys
Thr
Lys
Ala
Asp
Ala
Asp
Ala
Glu
Ala
Glu
Arg
STOP STOP A
Arg
Arg
Arg
Ser
Ser
Arg
Arg
Gly
Gly
Gly
DUAGUUAGDUAGUUAG
Gly
C
C
Α
C
А
C
Α
Transcribed Image Text:3. DNA polymerase made a mistake and added a C on the DNA template strand. In the space on the mRNA sequence below, write the added base. (Remember that the DNA template and mRNA are complementary. Mark the codons again and write the amino acid sequence beneath them. What do you observe? (5') CGUUACAAUGUAU CGCGCGGUACUCGGCAAAGUGCCCUGAAUAGAGUUGGUA 3' On this mRNA codon table, the first nucleotide in codon is to the left, the second is above, and third is to the right. 4. A mutation in the gene encoding the aminoacyl tRNA synthetase for valine (VAL) causes the tRNA binding site to have the wrong shape. In its new (mutant) shape, the enzyme can bind tRNAs for VAL and leucine (LEU). (not at the same time but enzymes work fast and there are lots of them) a. In cells with this mutant tRNA synthetase, will the protein product of translation match the original one you deduced in question 2? circle one: (YES / NO. ) b. If not, how does it differ? c. How many different version of the short polypeptide will exist in this mutant cell? U U Phe C Ser Alle lle lle Met G Val Val Val Val A Tyr Tyr Cys G Cys Phe Ser Leu Ser Leu Ser STOP Trp C Leu Pro His Leu Pro His Leu Pro Gin Leu Pro Gin Thr Asn Thr Asn Thr Lys Thr Lys Ala Asp Ala Asp Ala Glu Ala Glu Arg STOP STOP A Arg Arg Arg Ser Ser Arg Arg Gly Gly Gly DUAGUUAGDUAGUUAG Gly C C Α C А C Α
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