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- 1-The method of sterilizations we used in the tissue culture lab a. Fumigation b. Filtration C. Autoclave d. Sun light1. Describe and give the principle of each equipment used for sterilization in microbiology. (refer to equipment drawn only)1. Give the method of sterilization of these laboratory apparatuses how they are sterilized; inoculating loop, glasswares. culture media, skin, and milk. Please cite where you got the information. Thank you
- 4. All the following can be autoclaved EXCEPT: a) Biological material b) Glass ware c) Radioactive material d) Broth and gel media for growing bacteria5. The autoclave raises the atmospheric pressure to _____ : a) 10 psi b) 15 psi c) 20 k joules d) 25 watts6. In the experiment with serial dilutions, the plates which should be used for the final count for colonies should have a range of _______ CFU in order to have a reliable estimate: a) <10 b)10-15 c) 15-20 d) none of the above1. What is the hazard of the spattering tendency in heating a wire loop with specimens over a flame? 2. Why is heat a highly effective sterilizing agent?3. Which of the three methods of heat sterilization is the most appropriate and practical to use in sterilizing antibiotic solutions? (Direct flame, Dry heat, Moist heat)3. What is the difference between common rich media and defined media?
- The Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization?3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distille2. A mannitol-salt agar plate was inoculated with these bacteria and is shown below. A. What type of organisms grow on this medium? B. Based on the reaction below, what can you say about the organism derived from the patient's abscess?2) A pure culture A) is sterile. B) is a population of identical cells. C) is made of a clearly defined chemical medium. D) contains one microbial cell.
- 9. The process in which all organisms and pathogens are destroyed is called ------. a) Disinfection b) Sterilization c) Antisepsis d) Pasteurization1. The technique used in bacteria for endospore staining can also be used to observe fungal spores. Select one: True False 2. Which of the following statements is/are TRUE? a. Agar does not provide nutrients to microorganisms. b. Peptone, pork extracts, and yeast extracts provide nutrients to microbes. c. Tap water must be used for preparing culture media. d. A & B are correct. e. B & C are correct Clear my choice 3. Motility of microorganisms is BEST observed with a a. Flagellar stain b. Hanging drop preparation c. Solid medium d. Negative stain e. Streak plate Clear my choice1. If you are a thermophilic endospore forming bacterium what would be the appropriate and economically wise method for your preservation? Which culture collection centers will you select and why?