3. Explain why catabolite repression is used in regulating the lac operon anddescribe how it fine-tunes B-galactosidase synthesis.4. Compare the control of gene regulation in eukaryotes and prokaryotes in termsof:a. transcriptionb. regulatory mechanisms and its components

Name: 3. Explain why catabolite repression is used in regulating the lac op
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Description: 3. Explain why catabolite repression is used in regulating the lac operon anddescribe how it fine-tunes B-galactosidase synthesis.4. Compare the control of gene regulation in eukaryotes and prokaryotes in termsof:a. transcriptionb. regulatory mechan

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Biology

4. Compare the control of gene regulation in eukaryotes and prokaryotes in terms of: a. transcription b. regulatory mechanisms and its components

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 7TYU: Which of the following is characteristic of genes and gene regulation in both bacteria and...

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1. A)Explain the mechanism by which the lac operon is sensitive to glucose levels (presence and absence of glucose). B)If glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
1. Explain why catabolite repression is used in regulating the lac operon anddescribe how it fine-tunes B-galactosidase synthesis. 2.
1) A. What is the difference between a repressible vs, an inducible operon? B. Using diagrams and words, describe how the Lac Operon is regulated by different levels of lactose and glucose. Be sure to show what’s happening at the molecular level, including the roles of lactose, glucose the repressor protein, cAMP and CAP.
1) Assume you have an operon that is repressible. In this case, the rate of synthesis of repressor protein must depend on the level of a) co-repressor b) inducer c) both d) neither
1. If the above gene is one of the three structural genes of the lac operon that codes for theprotein/ enzyme responsible for breaking lactose into two molecules of simple sugars,a.) what triggers the activation of this gene?b.) what triggers the inactivation of this gene?c.) what substance is attached to the operator region of the operon in the absence ofactivator?d.) what gene is responsible for the synthesis of the substance used to attach in theoperator region in the absence of activator? e.) what substance is attached to the promoter region of the operon in this case?
1. What is the default state of the lac operon? a. LacI repressor is sitting on the operator sequence and blocks RNAP from transcribing lacZYA. b. LacI repressor is not in a correct shape to bind to the operator sequence. c. LacI repressor is not produced in the absence of lactose. d. Allolactose is sitting on the operator sequence and blocks RNAP from transcribing lacZYA.
1. How does induction control enzyme synthesis using lactose operon? 2. Describe how level of a key metabolite [tryptophane] controls the gene function using tryptophane operon
1. a)how is it possible for such drugs to selectively kill bacterial cells and not our own cells? b)Provide an example of post-translational regulation of protein activity and explain the advantage of regulating each protein/process at the post-translational level instead of the transcriptional level.
2) Name and describe two types of constitutive mutants of the lactose operon.
1. Assuming that the level of glucose is low, a mutation in the repressor associated with the lac operon of E. coli which prevents binding of the repressor to lactose should result in: a. constitutive expression of the lac operon genes b. lack of expression or refuced expression of the lac operon genes under all circumstances c. expression of the genes only when lactose is present d. expression of the genes only when lactose is absent e. lack of expression of the lac operon genes, resulting in an increase in lactose production
1. a) If glucose is not available and lactose is absent from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon? b)If glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon? b)
1. Describe which enzymes are required for lactose and tryptophan metabolism inbacteria when lactose and tryptophan, respectively, are (a) present and (b)absent. 2. Contrast positive versus negative regulation of gene expression. Describe therole of the repressor in an inducible system and in a repressible system.