1. Why is NSS ideal for performing the process of "washing" or "elution"? 2. Why is it necessary to wash the red cells with NSS three times prior to their use? 3. What is the importance of preparing a red cell suspension in the laboratory prior to performing the following procedure? 3.1 Blood Typing 3.2 Coomb's Test

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Chapter9: Cpt Evaluation And Management
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5. Using the following formula, complete the table in preparing red cell suspension with the desired
concentration. Show your computations below.
% red cell suspension = Amount of washed packed red blood cells
Total Volume
x 100
Total volume = amount of washed PRBC + amount of NSS to be added
Total Volume
Amount of washed
packed red blood cells
0.10 mL
0.15 mL
Solution:
0.20 mL
0.10 mL
Desired %
3%
3%
2%
3mL
5mL
Amount of NSS to be
added
9.80mL
Transcribed Image Text:5. Using the following formula, complete the table in preparing red cell suspension with the desired concentration. Show your computations below. % red cell suspension = Amount of washed packed red blood cells Total Volume x 100 Total volume = amount of washed PRBC + amount of NSS to be added Total Volume Amount of washed packed red blood cells 0.10 mL 0.15 mL Solution: 0.20 mL 0.10 mL Desired % 3% 3% 2% 3mL 5mL Amount of NSS to be added 9.80mL
ANSWERS TO QUESTIONS ON
LABORATORY ASSAY NO.3
1. Why is NSS ideal for performing the process of "washing" or "elution"?
2. Why is it necessary to wash the red cells with NSS three times prior to their use?
3. What is the importance of preparing a red cell suspension in the laboratory prior to performing the
following procedure?
3.1 Blood Typing
3.2 Coomb's Test
4. List the possible sources of error in the preparation of Red Blood Cell suspension?
Transcribed Image Text:ANSWERS TO QUESTIONS ON LABORATORY ASSAY NO.3 1. Why is NSS ideal for performing the process of "washing" or "elution"? 2. Why is it necessary to wash the red cells with NSS three times prior to their use? 3. What is the importance of preparing a red cell suspension in the laboratory prior to performing the following procedure? 3.1 Blood Typing 3.2 Coomb's Test 4. List the possible sources of error in the preparation of Red Blood Cell suspension?
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 4. List the possible sources of error in the preparation of Red Blood Cell suspension?

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5. Using the following formula, complete the table in preparing red cell suspension with the desired
concentration. Show your computations below.
% red cell suspension = Amount of washed packed red blood cells
Total Volume
x 100
Total volume = amount of washed PRBC + amount of NSS to be added
Desired %
Amount of washed
packed red blood cells
0.10 mL
0.15 mL
Solution:
0.20 mL
0.10 mL
3%
3%
2%
Total Volume
3mL
5mL
Amount of NSS to be
added
9.80mL
Transcribed Image Text:I I I 5. Using the following formula, complete the table in preparing red cell suspension with the desired concentration. Show your computations below. % red cell suspension = Amount of washed packed red blood cells Total Volume x 100 Total volume = amount of washed PRBC + amount of NSS to be added Desired % Amount of washed packed red blood cells 0.10 mL 0.15 mL Solution: 0.20 mL 0.10 mL 3% 3% 2% Total Volume 3mL 5mL Amount of NSS to be added 9.80mL
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