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4. The "Gibbs Reflective Cycle" be explained. In each phase of the reflective cycle, provide an example.?
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- 1 . What is the absorption peak(s) of bromocresol green? Make sure to write down the wavelength at which the absorption peak(s) occur. Include units, please 2.Are the absorption spectrums and peak(s) for methyl orange and bromocresol green the same? Why or why not?2. What feature(s) of proteins are responsible for UV absorbance at 280 nm?4. What are zwitterions? Do they interfere with the different color reaction tests?
- 3. Discuss the advantage and disadvantage of Wijs method of iodine value determination. What non-titrimetric method can be used to determine the iodine value of edible oil? Discuss its basic principles and general methods involved.8. What are the products obtained when coconut oil is heated with NaOH? Coconut oil + NaOH → ? + ?1.Would you expect a change in column temperature to have an effect on a reversed-phase chromatographic separation of a steroid mixture?
- 6. Which of the molecules below exhibits the following UV/Vis absorption spectrum? Assign the peaks in spectrum. Explain the answer.1. A prescription calls for 50 mg of chlorpheniramine maleate. Using a prescription balance with a sensitivity requirement of 6 mg, explain how you would obtain the required amount of chlorpheniramine maleate with an error not greater than 5%.Explain the "Gibbs Reflective Cycle". Give example in each step of the reflective cycle.
- 1. Why does one see bromocresol green as indigo in color? You may need to do some online research as to why we see color in order to answer this question appropriately. Do not research the effect that has pH has on the color of bromocresol green. 2. What blank would be used to standardize this spectrophotometer6. The Worthington's manual reports two extinction coefficients for lysozyme. Corvert one into the other. What is the absorbance at 280 nm of a 0.1 mg/mL lysozyme solution? Use both coefficients to estimate the result1. What color(s) does bromocresol green absorb the best? 2. What wavelength(s) does bromocresol green absorb the least? Include units,please. 3. What color(s) does bromocresol green absorb the least?