41) Dideoxyribonucleotides (ddNTPs) are unusual in both the 2' and 3' hydroxyl groups have been aced with protons. A lab technician who was cking the lab freezer with nucleotide vials accidentally cked the dCTP bin with ddCTP vials. How would this or affect an in vitro DNA replication reaction? DNA replication would be impossible Replication will proceed normally until the first guanosine nucleotide is reached. Replication will stop without cytosine being inserted into the daughter strand Replication will proceed normally until the first guanosine residue is reached. The ddCTP will be inserted, then replication will stop.

Biology 2e
2nd Edition
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter14: Dna Structure And Function
Section: Chapter Questions
Problem 17RQ: Which of the following is not a true statement comparing prokaryotic and eukaryotic DNA replication?...
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(Q141)
Dideoxyribonucleotides (ddNTPs) are unusual in
that both the 2' and 3' hydroxyl groups have been
replaced with protons. A lab technician who was
stocking the lab freezer with nucleotide vials accidentally
stocked the dCTP bin with ddCTP vials. How would this
error affect an in vitro DNA replication reaction?
DNA replication would be impossible
Replication will proceed normally until the first guanosine
nucleotide is reached. Replication will stop without cytosine
being inserted into the daughter strand
Replication will proceed normally until the first guanosine
residue is reached. The ddCTP will be inserted, then
replication will stop.
Replication will proceed normally until the first cytosine base
is reached in the parent strand, at which point replication
will terminate
DNA replication will be totally unaffected
Transcribed Image Text:(Q141) Dideoxyribonucleotides (ddNTPs) are unusual in that both the 2' and 3' hydroxyl groups have been replaced with protons. A lab technician who was stocking the lab freezer with nucleotide vials accidentally stocked the dCTP bin with ddCTP vials. How would this error affect an in vitro DNA replication reaction? DNA replication would be impossible Replication will proceed normally until the first guanosine nucleotide is reached. Replication will stop without cytosine being inserted into the daughter strand Replication will proceed normally until the first guanosine residue is reached. The ddCTP will be inserted, then replication will stop. Replication will proceed normally until the first cytosine base is reached in the parent strand, at which point replication will terminate DNA replication will be totally unaffected
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