5. The MLT performs patient testing against specific reagent anti-sera and obtains the following results: Anti-E Anti-e Anti-D 4+ Anti-c 3+ 0 3+ Anti-C 3+ a) What is this patient's Rh phenotype?
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- 1. Give the advantages of using tube method over the slide method. Why do you have to incubate the tube method in Rh typing? 2. Give the clinical importance of determining the Rh status of a patient? 3. Define HDN and explain the effect of Rh(D) antigen. What is the preventive measure of preventing D-negative mothers from forming anti-D? 4. What are the symptoms in Rh-mediated hemolytic transfusion reactions?1. What is the purpose of Rh view bow? What is the optimum temperature for detection of Rh antigen? 2. Give the different types of Rh antigen typing reagents accdg. to the sources. Explain each.An A negative mother (gravida 5, para 5) has just delivered an A positive newborn. Her antibody screen is positive and a panel shows the antibody is anti-D. The anti-D titer is 8. The child has a positive DAT. How could you determined how many doses of Rh immune globulin should be given to this mother?
- 1. How will you differentiate VDRL from RPR Card test? 2. Give the composition of VDRL reagent and their function.In some cases of weak D antigen testing, the interpretation of results can reveal that the person tested is somewhat D+ and D- at the same time. How is this possible? What is/are the implication/s of this Rh phenotyping result in the blood donation and transfusion of the said person?1.In radioimmunoassay the following reagents (parameters) are constant except A. Antibody (antiserum) B. Radiolabeled hormone (tracer) or (hot) C. Unlabeled hormone (cold) D. A and C E. None of the above
- 4. The function of allelic exclusion is? a. increase the magnitude of antibodies b. increase the affinity of antibodies c. increase the specificity of antibodies d. increase the diversity of antibodies e. increase sensitivity of antibodies1. What is/are the importance of testing Rh blood type in the laboratory? 2. Why is Rh system determination essential to pregnant and childbearing women? 3. What are the principal Rh antigens?Explain the following:1. What is immunologic agent drugs? 2. What is the importance of immunologic agent drugs to immunocompromised patients?
- 5. Describe the main procedures used in the manufacture of vaccines.4 If you are working in a diagnostic lab, what will be your choice among Monoclonal or Polyclonal Antibodies method for detection of specific pathogen? Give sufficient justification for selection as well as rejection of any given methods.1. Discuss how immunoassays differ from precipitation and agglutination reactions. 2. Differentiate between Direct and Indirect ELISA.