7. We can estimate the relative mobility of a population of molecules along the surface of a living cell by fluorescently labeling the molecules of interest, bleaching the label in one small area using laser, and then measuring the speed of signal recovery as molecules migrate back into the bleached area. What is this method called? What is its principle? (This technique that shows that proteins move along the membrane plane in lipid "rafts" exemplified on Fig. 11-34) A) SDS-PAGE B) western blot C) Confocal microscopy D) FRAP Answer: Explanation:

7. We can estimate the relative mobility of a population of molecules along the surface of a living cell by fluorescently labeling the molecules of interest, bleaching the label in one small area using laser, and then measuring the speed of signal recovery as molecules migrate back into the bleached area. What is this method called? What is its principle? (This technique that shows that proteins move along the membrane plane in lipid "rafts" exemplified on Fig. 11-34) A) SDS-PAGE B) western blot C) Confocal microscopy D) FRAP Answer: Explanation:

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter4: Cell Structure

Section: Chapter Questions

Problem 25CTQ: In your everyday life, you have probably noticed that certain instruments are ideal for certain...

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1. Working as an engineer in the R&D section of a biotech industry, you are asked to select a membrane to concentrate a solution containing a protein with a molecular weight of 200 kDa; Fortunately, it has three types of membrane in the cellar: one for MF, one for UF and one for RO. Which one would you choose to perform the required service? Could you establish some characteristics of the chosen membrane?
2. Unlike soluble, cytosolic proteins, transmembrane proteins are more difficult to purify, Which of the following substances is most used to help purify a transmembrane protein? a. high salt solution b. sucrose c. detergent d. ethanol e. glucose
6. A carrot, when dried out, becomes soft. There is a way to make it firm and crisp. Explain how and why, using the following guidelines. a) Draw and label a representative cell of the carrot, in its dried state. Be precise in your drawing. b)Describe the type of extracellular solution that is appropriate to add to make the desired change (name and define what type of solution, the net movements of solvent and solutes, etc.). c)Draw a representative cell of the carrot, in its possible state after the change. Represent the movement of water using an arrow to indicate what has happened.
1. The chains of several cell membrane-bound proteins wind back and forth through the cell membrane, such that some parts of the protein structure are extra-cellular, some parts are intra-cellular and some parts lie within the cell membrane. Do you think the primary structure of a protein can help in distinguishing the portions of a membrane protein embedded within the cell membrane from those that are not? Justify your answer.
1. Draw the structure of phosphatidic acid. Circle the polar parts of the molecule and box the non-polar parts of the molecule. 2.Based on the structure why would this molecule be a major building block for glycerophospholipids that make up the cell membrane? In your answer include which part of the molecule would be inside the membrane and which one out and why. 3. What specific “effect”would drive your answer to 2?
17. The transition temperature of a membrane bilayer is determined directly or indirectly a. the ability of lipid molecules to be packed together b. whether the fatty acid chains of the lipids are saturated or unsaturated c. the extent to which the fatty acid chains of the lipids contain double bonds d. the length of the fatty acid chains. e. All of the above are correct answers.
1. What are the forces that maintain structural integrity of biological membranes and explain how each force mentioned is formed in membrane lipids.
A scientist compares the plasma membrane composition of an animal from the Mediterranean coast with one from the Mojave Desert. Which hypothesis is most likely to be correct? The cells from the Mediterranean coast animal will have more fluid plasma membranes The cells from the Mojave Desert animal will have a higher cholesterol concentration in the plasma membranes The cells’ plasma membranes will be indistinguishable The cells from the Mediterranean coast animal will have a higher glycoprotein content, while the cells from the Mojave Desert animal will have a higher lipoprotein content.
Which of the following statements about proteins is correct? a. Proteins are transported to the rough ER for use within the cell. b. Lipids and carbohydrates are added to proteins by the Golgi complex. c. Proteins are transported directly into the cytosol for secretion from the cell. d. Proteins that are to be stored by the cell are moved to the rough ER. e. Proteins are synthesized in vesicles.
Figure 3.23 Which categories of amino acid would you expect to find on the surface of a soluble protein, and which would you expect to find in the interior? What distribution of amino acids would you expect to find in a protein embedded in a lipid bilayer?
3. Why do we need to study the four levels of protein structure? Cite its practical use to our day-to-day life.
1. In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored? 2. How does SDS-PAGE separate proteins and peptides from each other? Explain. 3. Explain the Donnan Membrane Phenomenon. Why is it important for the homeostasis of the cell?