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A 12 kb linear DNA fragment is subject to single or double RE digest and agarose electrophoresis, to yield the gel profile shown below. The first lane contains the size ma (M). 4. M Undigested EcoRI control PvuII ECORI + PvuII 12000 bp 9000 bp - 6000 bp | - 5000 bp - 4000 bp - - 2000 bp

A 12 kb linear DNA fragment is subject to single or double RE digest and agarose electrophoresis, to yield the gel profile shown below. The first lane contains the size ma (M). 4. M Undigested EcoRI control PvuII ECORI + PvuII 12000 bp 9000 bp - 6000 bp | - 5000 bp - 4000 bp - - 2000 bp

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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4. A 12 kb linear DNA fragment is subject to single or double RE digest and agarose gel electrophoresis, to yield the gel profile shown below. The first lane contains the size marker (M). M Undigested EcoRI control PvuII ECORI + PvuI 12000 bp 9000 bp 6000 bp 5000 bp 4000 bp 2000 bp 1000 bp Explain how the name of the enzyme ECORI is derived. How many sites are there for EcoRI and PvuII respectively on this DNA fragment? b) c) Use the sizes of the DNA bands on the gel to compile a restriction enzyme map of the DNA fragment. Indicate the positions of the restriction enzymes sites for EcoRI and PvuII on the map.
Transcribed Image Text:4. A 12 kb linear DNA fragment is subject to single or double RE digest and agarose gel electrophoresis, to yield the gel profile shown below. The first lane contains the size marker (M). M Undigested EcoRI control PvuII ECORI + PvuI 12000 bp 9000 bp 6000 bp 5000 bp 4000 bp 2000 bp 1000 bp Explain how the name of the enzyme ECORI is derived. How many sites are there for EcoRI and PvuII respectively on this DNA fragment? b) c) Use the sizes of the DNA bands on the gel to compile a restriction enzyme map of the DNA fragment. Indicate the positions of the restriction enzymes sites for EcoRI and PvuII on the map.
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