Homework Help is Here – Start Your Trial Now!
Science
Advanced Physics
(a) A medical researcher is using the uniform electric field (E = 2400 NC-¹) generated by an electrophoresis setup to isolate a charged protein of interest (q = +160e) from an uncharged patient sample. As shown in panel (A), when the patient sample is loaded between the positive and negative terminal, the charged protein of interest migrates 12 cm toward the negative terminal.

(a) A medical researcher is using the uniform electric field (E = 2400 NC-¹) generated by an electrophoresis setup to isolate a charged protein of interest (q = +160e) from an uncharged patient sample. As shown in panel (A), when the patient sample is loaded between the positive and negative terminal, the charged protein of interest migrates 12 cm toward the negative terminal.

icon
Related questions
Question
Question 5 Electromagnetic phenomena and spectroscopy. (a) A medical researcher is using the uniform electric field (E = 2400 NC-¹) generated by an electrophoresis setup to isolate a charged protein of interest (q = +160e) from an uncharged patient sample. As shown in panel (A), when the patient sample is loaded between the positive and negative terminal, the charged protein of interest migrates 12 cm toward the negative terminal. (A) (B) €€€ (ii) (iii) (iv) (c) +160e -0 (1) (ii) (iii) 12 cm = protein of interest = patient sample Intensity (a.u.) 100 80 60 40 20 ol 450 Excitation spectrum (b) Migration of the charged protein of interest was monitored by staining the patient sample with a fluorescent dye that was specific for this biological molecule. The excitation and emission spectrum of this fluorescent dye are presented in panel (B). (1) Emission spectrum 500 550 600 Wavelength (nm) How much work has the electric field done on the protein of interest? What is the protein of interest's change in electric potential energy? If the medical researcher wanted the charged protein of interest to migrate further away from the uncharged patient sample, how could they achieve this? What is the strength of the mass spectrometer's magnetic field? Is the magnetic field vector pointing into the page or out of the page? What is the mass of component A? 650 What wavelength of light (A) could be used to excite the fluorescent dye highlighting the protein of interest within the electrophoresis gel? What wavelength of light (A) could be used to detect the resulting fluorescence? Calculate the photon energy associated with the wavelength of light (A) that you suggest could excite the fluorescent dye. Briefly describe why the emission spectrum of the fluorescent dye in panel (B) occurs at longer wavelengths of light (A) compared to its excitation spectrum. The extracted protein of interest is then subjected to mass spectrometry for characterisation. As depicted in panel (C), when the protein enters the magnetic field of the mass spectrometer with a velocity of 9 x 104 ms-1, two singly ionised components (A and B) are detected 8 cm and 10 cm to the left of the point of entry. The mass of component B is reported to be 7 x 10-23 kg. (C) 2 cm A B 0024 8 cm Protein of interest
Transcribed Image Text:Question 5 Electromagnetic phenomena and spectroscopy. (a) A medical researcher is using the uniform electric field (E = 2400 NC-¹) generated by an electrophoresis setup to isolate a charged protein of interest (q = +160e) from an uncharged patient sample. As shown in panel (A), when the patient sample is loaded between the positive and negative terminal, the charged protein of interest migrates 12 cm toward the negative terminal. (A) (B) €€€ (ii) (iii) (iv) (c) +160e -0 (1) (ii) (iii) 12 cm = protein of interest = patient sample Intensity (a.u.) 100 80 60 40 20 ol 450 Excitation spectrum (b) Migration of the charged protein of interest was monitored by staining the patient sample with a fluorescent dye that was specific for this biological molecule. The excitation and emission spectrum of this fluorescent dye are presented in panel (B). (1) Emission spectrum 500 550 600 Wavelength (nm) How much work has the electric field done on the protein of interest? What is the protein of interest's change in electric potential energy? If the medical researcher wanted the charged protein of interest to migrate further away from the uncharged patient sample, how could they achieve this? What is the strength of the mass spectrometer's magnetic field? Is the magnetic field vector pointing into the page or out of the page? What is the mass of component A? 650 What wavelength of light (A) could be used to excite the fluorescent dye highlighting the protein of interest within the electrophoresis gel? What wavelength of light (A) could be used to detect the resulting fluorescence? Calculate the photon energy associated with the wavelength of light (A) that you suggest could excite the fluorescent dye. Briefly describe why the emission spectrum of the fluorescent dye in panel (B) occurs at longer wavelengths of light (A) compared to its excitation spectrum. The extracted protein of interest is then subjected to mass spectrometry for characterisation. As depicted in panel (C), when the protein enters the magnetic field of the mass spectrometer with a velocity of 9 x 104 ms-1, two singly ionised components (A and B) are detected 8 cm and 10 cm to the left of the point of entry. The mass of component B is reported to be 7 x 10-23 kg. (C) 2 cm A B 0024 8 cm Protein of interest
Expert Solution
steps

Step by step

Solved in 3 steps with 7 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer