A column 3.00 cm in diameter and 32.6 cm long gives adequate resolution of a 72.4-mg mixture of unknowns, initially dissolved in 0.500 mL. (a) If you wish to scale down to 10.0 mg of the same mixture with minimum use of chromatographic stationary phase and solvent, what length and diameter column would you use? (b) In what volume would you dissolve the sample? (c) If the flow rate in the large column is 1.85 mL/min, what should be the flow rate in the small column?

A column 3.00 cm in diameter and 32.6 cm long gives adequate resolution of a 72.4-mg mixture of unknowns, initially dissolved in 0.500 mL. (a) If you wish to scale down to 10.0 mg of the same mixture with minimum use of chromatographic stationary phase and solvent, what length and diameter column would you use? (b) In what volume would you dissolve the sample? (c) If the flow rate in the large column is 1.85 mL/min, what should be the flow rate in the small column?

Solid Waste Engineering

3rd Edition

ISBN:9781305635203

Author:Worrell, William A.

Publisher:Worrell, William A.

Chapter5: Separation Processes

Section: Chapter Questions

Problem 5.32P

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